利用BSMVAgro/LIC进行小麦条锈菌耐高温相关基因功能分析的互作体系构建

Development of an interactive system for functional analysis of high-temperature tolerance-related genes of wheat stripe rust pathogen by using BSMV Agro/LIC

为利用HIGS技术研究条锈菌耐高温胁迫相关基因的功能,需要构建小麦品种与条锈菌及大麦条纹花叶病毒Barley stripe mosaic virus(BSMV)亲和互作体系。本研究选用20个常温下(10℃)高感条锈病的小麦品种和22个耐高温条锈菌菌株,进行高温(21℃)条件下接种亲和性分析,发现11个品种高感条锈病、3个菌株耐高温性稳定且毒性谱较宽。在此基础上,利用含有小麦TaPDS基因片段的重组BSMV侵染上述候选小麦品种,确定‘Local Red'等6个品种与BSMV亲和程度符合试验要求。筛选出小麦品种‘Local Red'与菌株15335-2、15323-4等组成的最佳互作组合,适合利用BSMV Agro/LIC方法开展小麦条锈菌耐高温相关基因功能的研究。

In order to characterize the biological functions of the high-temperature tolerance-related genes of Puccinia striiformis f.sp.tritici（Pst）by using HIGS method,it is necessary to develop a compatible interactive system containing wheat variety and Pst pathogen and Barley stripe mosaic virus（BSMV）.In this study,20 varieties（highly susceptible when inoculated at 10℃）were inoculated with 22 high-temperature-tolerant isolates at 21℃ and their compatibility levels were evaluated,and we found 11 highly susceptible varieties among the tested ones and 3 isolates with a highly stable level of high-temperature tolerance and a wider virulence spectrum.Based on the results,6 varieties such as‘Local Red＇were compatible with the BSMV strain containing aTaPDSfragment.Therefore,the combinations of the variety‘Local Red＇with the isolates like 15335-2 and 15323-4 were the best interactive systems for characterizing high temperature-tolerance-related genes of Pst with BSMV Agro/LIC.