纳米αFe2O3对牙髓干细胞增殖及骨向分化和矿化能力的影响

Effects of nano αFe_2O_3 on proliferation,osteogenic differentiation and mineral synthesis of dental pulp stem cells

对纳米αFe_2O_3进行二巯基丁二酸表面修饰,采用透射电子显微镜(TEM)观察其形貌,利用振动样品磁强计(VSM)检测其磁学性能.配置含纳米αFe_2O_3浓度为10μg/m L的培养基培养牙髓干细胞,以不添加纳米αFe_2O_3的培养基为对照组.采用荧光显微镜观察纳米αFe_2O_3组与常规培养基组的细胞形态,以CCK-8检测细胞增殖情况,碱性磷酸酶(ALP)试剂盒检测细胞ALP活性,并进行茜素红染色以检验其矿化能力.结果表明,所用纳米αFe_2O_3为棒状,尺寸约10nm×90 nm,VSM结果显示其没有磁性.纳米αFe_2O_3培养基中牙髓干细胞的形态和增殖情况与常规培养基中情况没有差异,但是碱性磷酸酶活性和矿化能力则显著提高(p<0.05).由此说明,纳米αFe_2O_3对牙髓干细胞的形态和增殖没有影响,但却会促进其向成骨方向分化.

Nano αFe2O3 is surface modified with dimercaptosuccinic acid（ DM SA）. Its morphology is observed by transmission electronic microscopy（ TEM）,and its magnetic property is detected by vibration sample magnetometer（ VSM）. The media w ith 10 μg/m L nano αFe2O3 is used to culture dental pulp stem cells（ DPSCs） by using the media without nano αFe2O3 as the control. The morphology of DPSCs in the nano αFe2O3 group and the control group is detected by fluorescent stain. The cell proliferation is detected by CCK-8,and the cell alkaline phosphatase activity（ ALP） is measured by a ALP kit. Alizarin red staining（ ARS） is used to detect the mineral synthesis. The results show that nano αFe2O3 is spindle with about 10 nm × 90 nm in dimension. The VSM results prove that nanoαFe2O3 is nonmagnetic. No significant difference in cell morphology and proliferation betw een the nano αFe2O3 group and the normal media group is detected. However,both ALP activity and mineralization formation of cells are significantly increased（ p〈0. 05）. Therefore,nano αFe2O3 does not affect the cell morphology and proliferation,but promotes the osteogenic differentiation of DPSCs.