口腔鳞状细胞癌内c-fos、p16表达量与细胞周期、细胞侵袭的相关性

The correlation of c-fos and p16 expression in oral squamous cell carcinoma with cell cycle and cell invasion

目的:研究口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)c-fos、p16表达量与细胞周期、细胞侵袭的相关性。方法:选择2014年6月~2017年3月期间在枣矿集团中心医院接受手术切除治疗的口腔鳞状细胞癌患者作为研究的OSCC组,同期在枣矿集团中心医院拔出阻生齿且留取正常牙龈黏膜组织的患者作为对照组。留取OSCC组患者的病灶组织及对照组患者的正常组织,测定c-fos、p16及细胞周期分子、细胞侵袭分子的表达量。结果:OSCC组病灶组织中c-fos的mRNA表达量显著高于对照组(P<0.05),p16的mRNA表达量显著低于对照组(P<0.05),且c-fos的mRNA表达量与p16的mRNA表达量呈负相关(P<0.05);OSCC组病灶组织中Chk1、Chk2的mRNA表达量显著低于对照组(P<0.05),NEK2、CyclinD1、MALAT1、Periostin、β-catenin、Vimentin的mRNA表达量显著高于对照组(P<0.05);c-fos高表达的OSCC病灶组织中Chk1、Chk2的mRNA表达量显著低于c-fos低表达的OSCC病灶组织(P<0.05),NEK2、CyclinD1、MALAT1、Periostin、β-catenin、Vimentin的mRNA表达量显著高于c-fos低表达的OSCC病灶组织(P<0.05)。结论:口腔鳞状细胞癌内c-fos的高表达以及p16的低表达能够加速细胞周期、促进细胞侵袭。

Objective:To study the correlation of c-fos and p16 expression in oral squamous cell carcinoma with cell cycle and cell invasion.Methods:Patients with oral squamous cell carcinoma who underwent surgical resection in Zaozhuang Mining Group Central Hospital between June 2014 and March 2017 were selected as OSCC group of the research,and patients who received impacted tooth extraction and provided normal gingival mucosa tissue in the Zaozhuang Mining Group Central Hospital during the same period were selected as the control group.The lesion tissue and normal tissue were obtained from OSCC group and control group respectively to determine the expression of c-fos,p16,cell cycle molecules and cell invasion molecules.Results:c-fos mRNA expression in lesion tissue of OSCC group was significantly higher than that of control group,p16 mRNA expression was significantly lower than that of control group,and the c-fos mRNA expression was negatively correlated with p16 mRNA expression;Chk1 and Chk2 mRNA expression in lesion tissue of OSCC group were significantly lower than those of control group whereas NEK2,CyclinD1,MALAT1,Periostin,β-catenin and Vimentin mRNA expression were significantly higher than those of control group;Chk1 and Chk2 mRNA expression in OSCC lesion tissue with high c-fos expression were significantly lower than those in OSCC lesion tissue with low c-fos expression whereas NEK2,CyclinD1,MALAT1,Periostin,β-catenin and Vimentin mRNA expression were significantly higher than those in OSCC lesion tissue with low c-fos expression.Conclusion:The high expression of c-fos and the low expression of p16 in oral squamous cell carcinoma can accelerate cell cycle and promote cell invasion.