宫颈癌超声弹性成像参数与癌细胞恶性生物学行为的相关性研究

The correlation between the ultrasonic elastography parameters of cervical cancer and the malignant biological behavior of cancer cells

目的:探讨宫颈癌超声弹性成像参数与癌细胞恶性生物学行为的相关性。方法:180例在本院确诊并接受根治术治疗的宫颈癌患者作为研究对象,术中留取宫颈癌组织、癌旁组织标本,并冻存于液氮罐中备用。术前检测宫颈癌及癌旁组织的超声弹性应变比值(strain ratio),对比其中增殖、侵袭、自噬相关基因表达量的差异。采用Pearson检验评估宫颈癌strain ratio与癌细胞恶性生物学行为的相关性。结果:宫颈癌组织的strain ratio水平高于癌旁组织(P<0.05);增殖相关基因AEG-1、TRAF6、FoxM1mRNA的表达量高于癌旁组织(P<0.05),SFRP2、MST1、Bmi-1mRNA的表达量低于癌旁组织(P<0.05);侵袭相关基因ILK、Sox2、Twist、YB-1、Rab11 mRNA的表达量高于癌旁组织(P<0.05);自噬相关基因ARHI、Beclin1、PULK、PI3KC3 mRNA的表达量低于癌旁组织(P<0.05)。结论:宫颈癌超声strain ratio水平与癌细胞的增殖、侵袭、自噬活性均密切相关,与肿瘤整体恶性程度呈正相关。

Objective:To investigate the correlation between the ultrasonic elastography parameters of cervical cancer and the malignant biological behavior of cancer cells.Methods:A total of 180 patients with cervical cancer who were diagnosed and treated with radical operation in this hospital were selected as research subjects,and the cervical cancer tissue and adjacent tissue samples were collected during operation and stored in liquid nitrogen tanks.The ultrasonic elastography strain ratio of cervical cancer and adjacent tissues was detected before operation,and the differences in the expression of genes associated with proliferation,invasion and autophagy were compared.Pearson test was used to evaluate the correlation between the cervical cancer strain ratio and the malignant biological behavior of cancer cells.Results:The strain ratio level of cervical cancer tissue was higher than that of adjacent tissue(P<0.05);proliferation-related genes AEG-1,TRAF6 and FoxM1 mRNA expression were higher than those in adjacent tissue(P<0.05)whereas SFRP2,MST1 and Bmi-1 mRNA expression were lower than those in adjacent tissue(P<0.05);invasion-related genes ILK,Sox2,Twist,YB-1 and Rab11 mRNA expression were higher than those in adjacent tissue(P<0.05);autophagy-related genes ARHI,Beclin1,PULK and PI3 KC3 mRNA expression were lower than those in adjacent tissue(P<0.05).Conclusions:Ultrasonic strain ratio level of cervical cancer is closely related to the proliferation,invasion and autophagy activity of cancer cells,and is positively correlated with the overall malignancy of the tumor.