多普勒超声参数与子宫内膜癌病灶内血管新生、癌细胞生长的相关性

The correlation of Doppler ultrasound parameters with angiogenesis and cancer cell growth in endometrial cancer

目的:研究多普勒超声参数与子宫内膜癌病灶内血管新生、癌细胞生长的相关性。方法:选择在本院接受手术切除治疗的子宫内膜癌患者和子宫肌瘤患者作为研究对象,分别纳入研究的观察组和对照组。术前进行盆腔多普勒超声检查并测定子宫内膜病灶的血流参数阻力指数(RI)以及搏动指数(PI);手术切除后,取观察组患者的子宫内膜癌病灶组织以及对照组患者的正常子宫内膜组织,测定促增殖分子、促侵袭分子、血管新生分子的含量。结果:观察组患者子宫内膜癌病灶的PI和RI水平显著低于对照组,子宫内膜癌病灶组织中cIAP1、cIAP2、RLIP76、p-Pak4^(ser474)、EFEMP2、CXCR4、CXCR7、Snail、Septin-9、β-arrestin2、SOX2的蛋白含量显著高于对照组;子宫内膜癌病灶组织PI、RI的水平与cIAP1、cIAP2、RLIP76、p-Pak4^(ser474)、EFEMP2、CXCR4、CXCR7、Snail、Septin-9、β-arrestin2、SOX2的蛋白含量呈负相关。结论:子宫内膜癌病灶的多普勒超声参数PI和RI与病灶内血管新生、癌细胞生长密切相关。

Objective:To study the correlation of Doppler ultrasound parameters with angiogenesis and cancer cell growth in endometrial cancer.Methods:Patients with endometrial cancer and fibroids who accepted surgical resection in the Central Hospital of Enshi Autonomous Prefecture between March 2015and June 2017were selected as the research subjects and enrolled in the observation group and control group of the study respectively.The preoperative pelvic Doppler ultrasonography was done to determine the blood flow parameters resistance index(RI)and pulsatility index(PI)of the endometrial lesions;after surgical resection,the endometrial cancer lesion tissue was collected from the observation group and normal endometrial tissue was collected from the control group to determine the contents of pro-proliferation molecules,pro-invasion molecules and angiogenesis molecules.Results:PI and RI levels in endometrial cancer lesion of observation group were significantly lower than those of control group,and cIAP1,cIAP2,RLIP76,p-Pak4^(ser474),EFEMP2,CXCR4,CXCR7,Snail,Septin-9,β-arrestin2 and SOX2 protein levels in endometrial cancer lesion were significantly higher than those of control group;PI and RI levels in endometrial cancer lesion were negatively correlated with cIAP1,cIAP2,RLIP76,p-Pak4^(ser474),EFEMP2,CXCR4,CXCR7,Snail,Septin-9,β-arrestin2 and SOX2 protein levels.Conclusion:The Doppler ultrasound parameters of endometrial carcinoma lesion are closely related to the angiogenesis and cancer cell growth in the lesion.