FGF8、SHH、FOXL2基因在鸡胚成纤维细胞中的调控作用研究

The regulation of FGF8, SHH and FOXL2 in chicken embryonic fibroblast cells

为了研究SHH、FGF8和FOXL2三个基因在鸡和鹌鹑杂交禽胚胎早期发育中的作用,分别构建鸡SHH、FGF8和FOXL2三个基因共9个RNA干扰载体,构建FGF8基因和SHH基因的过表达载体;将干扰载体和过表达载体分别转染鸡胚成纤维细胞。结果表明:FGF8基因通过MAPK信号通路影响杂交种胚胎早期发育;SHH基因通过Hedgehog信号通路影响杂交种胚胎早期发育;FOXL2基因通过调节DMRT1、SOX9和CYP19等基因的表达变化来影响杂交种胚胎早期发育。本研究可为进一步研究雌性杂交种胚胎早期死亡的原因奠定理论基础。

To research the function mechanism of SHH, FGF8 and FOXL2 in embryologic development of chicken-quail hybrids. Nine RNA interference vectors of FGF8, SHH, FOXL2 genes, two overexpression vectors of FGF8, SHH genes were constructed successfully, respectively. These vectors were transferred into chicken embryonic fibroblast cells by liposomes transfection, respectively. The results showed that FGF8 maybe influence early embryologic development of hybrid by effecting MAPK signaling pathway; SHH maybe affect embryologic development through having effect on Hedgehog signaling pathway; FOXL2 influence early embryologic development by regulating the expression level of DMRT1, SOX9 and CYP19. The results would build the theory base for further study of death reason during early development of female hybrid embryos.