摘要
目的:通过对昏迷大鼠促醒研究,探索其可能的促醒机制。方法:100只SD大鼠随机分为假手术组、模型组、针刺组、醒脑静组和针刺+醒脑静组,用改良四血管阻塞法建立大鼠全颅缺血性脑损伤昏迷模型,给予各组不同干预后,通过免疫组化法观察各组脑内GABA、GLU、β-EP的表达,并测定其含量。结果:促醒组的GABA、β-EP表达的降低和GLU表达增高较假手术组和模型组均有统计学差异(P<0.05),其中针刺+醒脑静组表达减低(或增高)更为显著(P<0.01)。结论:针刺对全颅缺血性昏迷大鼠的促醒机制是通过减少抑制性神经递质GABA、β-EP表达和增加兴奋性神经递质GLU的表达,且针刺+醒脑静的联合应用促醒效果更佳。
Objective To explore the possible mechanism of awakening through the study on the coma rats. Methods： 100 SD rats were randomly divided into the control group, model group, acupuncture group, XNJ group and acupuncture plus XNJ group. Ischemic rats with brain injury and coma were established by improved four-vessel occlusion method. After intervention, the expression of GABA, GLU and β-EP in the brain was observed by immunohistochemistry, and the content of GABA, GLU and β-EP in the brain was determined. Results： There are statistic diferences of expression of GABA, β-EP, GLU between the a wakening groups and control group （P〈0.05）. The decrease of expression of GABA, β-EP and increase of expression of GLU were more significant in the acupuncture plus XNJ group. Conclusion： The mechanism of awakening unconscious rats model of ischemic by acupuncture in ischemic rats with coma was the decreased of expression of GABA, β-EP and increased of expression of GLU. Efcacy of refreshing static plus acupuncture plus XNJ on awakening is better.
出处
《中医临床研究》
2017年第34期7-11,共5页
Clinical Journal Of Chinese Medicine
