一种双通道检测硫化氢的近红外荧光探针的合成及性能研究

A Dual Channel Near-infrared Fluorescent Probe for Selective Detection of Endogenous Hydrogen Sulfides and its Application in Living Cells

研究开发一种能够快速、灵敏、选择性检测硫化氢(H2S)的荧光探针具有重要的生理学意义。本文报道了一种能够双通道检测H2S的近红外荧光探针。该探针由类罗丹明衍生物与醛基香豆素出发,通过Knoevenagel缩合反应得到,制备方法简单,稳定性好,背景荧光极弱,可快速、选择性的与硫化氢响应,表现为紫外光谱发生比率型变化并伴随约130 nm的红移,荧光光谱在662 nm与818 nm发生双通道增强。此外,其它小分子以及阴离子的存在对该检测体系均无干扰,可以用于活细胞内源性H2S的检测。

The development of probes for rapid, selective, and sensitive detection of the hydrogen sulfidesgreat importance in biological science. We report herea Near-infrared （NIR） fluorescent off-on probe fortive and sensitive detection of hydrogen sulfides （H2S） with dual channel signal changes. The probe was ssized by Knoevenagel condensation reaction coupling betweena rhodamine derivative anda coumarin aldThe probe showed a specific off-on response to H2S in aqueous solution with nanomolar LOD, and withoterference bya range of competing sulfocompounds and anions. Moreover, it can be used for imaging of ennous H2S in living cells.