HCV-RNA及ALT、AST联合检测在丙肝患者诊断治疗中的应用研究

Research on Application of HCV-RNA, ALT and AST in Diagnosis and Treatment of Patients with Hepatitis C

目的探讨和分析丙肝患者HCV-RNA及ALT、AST联合检测的重要价值。方法方便选取该院在2016年3月—2017年5月期间根据收诊对象的不同,将进行诊疗的93例丙肝患者分为观察组,选取同时期93名体检正常者确定为对照组,均进行血清取样并送检,测定HCV-RNA与抗-HCV的实际含量、以及ALT与AST的活性。结果随着HCV-RNA病毒实际含量的增加,抗-HCV的阳性率也愈高,最高可达100.00%,二者属于正相关。按照HCV-RNA病毒含量的实际测定情况,将患者划成以下3个组,即低中高病毒含量组。和对照组相对比,在病毒含量的出现上升时,丙肝患者实际的AST水平[最大值为(159.1±17.2)U/L]与ALT水平[最大值为(140.1±13.7)U/L]均显著上升(P<0.05)。和对照组进行对比,其余各组的AST/ALT比值[最大值为(1.62±0.14)U/L]都显著上升,且与病毒实际含量呈现正相关。结论联合检测有利于相关医疗人员及时知晓丙肝患者的肝脏炎性反应状态与HCV复制进程,对丙肝患者的诊治具有极大现实意义与临床价值。

Objective To study and analyze the application value of HCV-RNA, ALT and AST in diagnosis and treatment of patients with hepatitis C. Methods 93 cases of patients with hepatitis C admitted and treated in our hospital from March2016 to May 2017 were convenient selected as the observation group, while 93 cases of physical examination people at the same period were selected as the control group, and the serum was extracted for submission, and the practical contents of HCV-RNA and anti-HCV and activities of ALT and AST were measured. Results With the increase of practical content of HCV-RNA, the patients were divided into three groups, including the low, moderate and high virus contents group, the practical AST level and ALT level of patients with hepatitis C(maximum values were respectively(159.1±17.2)U/L and(140.1±13.7)U/L,of patients with hepatitis C obviously increased(P<0.05), and the ratio of AST/ALT in other groups[maximum value was(1.62±0.14)U/L] obviously increased, positively correlated with the practical content of viruses. Conclusion The combined test can help related medical staff know the liver inflammatory reaction stage and HCV replication process of patients with hepatitis C, which is of reality significance and clinical value to the diagnosis and treatment of patients with hepatitis C.