Th1/Th2细胞因子在初发型溃疡性结肠炎治疗前后的血清水平变化

The Changes of Serum Levels of Th1/Th2 Cytokines before and after the Treatment of Active Ulcerative Colitis

目的:观察溃疡性结肠炎(UC)治疗前后血清Th1/Th2细胞因子的水平变化。方法:将初发型UC患者58例作为UC组,选取同期健康人58例作为对照组,在治疗前及治疗3月、12月3个时间点检测血清中IL-2、IFN-γ、IL-4、IL-10表达水平。结果:治疗前,UC组患者血清IFN-γ、IL-10水平明显降低,IL-4水平明显升高,均有统计学差异(P<0.05)。治疗3月及12月,UC组IFN-γ水平仍明显低于对照组,但UC组前后无差异;IL-4水平明显高于对照组,且治疗12月IL-4水平较治疗3月时显著降低(P<0.05);IL-10水平明显低于对照组,但治疗12月IL-10水平较治疗3月时显著升高(P<0.05)。UC组与对照组治疗前后IL-2水平无差异性变化(P>0.05)。结论:我们推测UC活动期血清IFN-γ表达下调、IL-4表达上调,一定程度抑制Th1细胞免疫反应,抑制炎症进展。随着UC病情缓解,血清IL-10表达上调、IL-4表达下调,逐渐恢复Th1/Th2细胞平衡。

Objective： To observe the changes of serum levels of Th1/Th2 cytokin before and after the treatment of ulcerative colitis（ UC）. Methods： 58 patients with active UC were selected into UC group while58 healthy persons as control group. The expression of IL-2、IFN-γ、IL-4、IL-10 in serum were measured at the 3 different time points： before treatment,3 months and 12 months after treatment. Results： Before treatment,the expression of IFN-γ、IL-10 in serum decreased but IL-4 increased in UC group,with statistical differences（ P〈0.05）. 3 months and 12 months after treatment,the expression of IFN-γ in UC group was lower significantly than control group,but no statistical difference was found in UC group. The expression of IL-4 was higher significantly than control group,especially at the time point of 12 months after treatment（ P〈0.05）. The expression of IL-10 was lower than control group,but it increased significantly at the time point of12 months after treatment（ P〈0.05）. There was no statistical difference in the expression of IL-2 between the two groups（ P〈0.05）. Conclusion： It was suggested that the expression of IFN-γ decreased and IL-4 increased in serum during active UC,would inhibit the Th1 cells immune response to limit inflammatory process.During the remission of UC,the expression of IL-4 decreased and IL-10 increased would keep the balance of Th1/Th2 cells gradually.