伏隔核区Jak/Stat3抑制剂注射调控布氏田鼠的能量代谢

Nucleus Accumbens Microinjection Jak/Stat3 Inhibitor Regulate Energy Metabolism of Lasiopodomys brandtii

目的探究抑制布氏田鼠Lasiopodomys brandtii伏隔核区leptin-Stat3(瘦素-Stat3)细胞信号通路对其能量代谢以及体质量的影响。方法在布氏田鼠伏隔核区定点注射Jak/Stat3抑制剂(WP1066),使用TSE代谢笼系统检测布氏田鼠的基础代谢,并随时记录布氏田鼠的体质量。结果在伏隔核区定点注射Jak/Stat3抑制剂后,布氏田鼠伏隔核区P-Stat3表达量以及瘦素表达水平明显下降(P<0.05),而血清瘦素浓度以及血糖浓度没有显著变化(P>0.05)。TSE代谢笼实验检测发现,实验组布氏田鼠总能量消耗显著降低(P<0.05)、呼吸交换率显著增加(P<0.05)、总耗氧量显著降低(P<0.05)、总自主运动量显著减弱(P<0.05)、总饮水量显著增加(P<0.05)、体质量显著增加(P<0.05)。结论调控伏隔核区的leptin-Stat3信号通路能够调控基础代谢,进而在10 d内改变布氏田鼠体质量。

Objective To explore the effect of impaired leptin-Stat3 in nucleus accumbens on the metabolism and body mass of Lasiopodomys brandtii. Methods Inhibitor of Jak/Stat3（ WP1066） was injected into the nucleus accumbens of L. brandtii,and then the basic metabolism was tested by TSE metabolic cage system followed by monitoring the body mass.Results Jak/Stat3 could significantly decrease the levels of P-Stat3 and leptin in nucleus accumbens（ P 〈 0. 05）. However,there was no significant difference in the levels of serum leptin and blood glucose（ P 〈 0. 05）. The result of TSE metabolic cage system showed that the energy expenditure,cumulative O2 consumption and cumulative locomotor activity decreased significantly,while the respiratory exchange rate and cumulative drink increased significantly in experimental group（ P 〈 0. 05）. Moreover,the average body mass of experimental group increased significantly（ P 〈 0. 05）. Conclusion Inhibition of leptin-Stat3 pathway in nucleus accumbens of L. brandtii can influence energy metabolism and body mass of L. brandtii in 10 days.