低强度脉冲超声对环磷酰胺致卵巢早衰大鼠卵巢GDF9和BMP15表达的影响

Effects of low intensity pulsed ultrasound on expression of GDF9 and BMP15 in ovaries of premature ovarian failure rats induced by cyclophosphamide

目的探讨低强度脉冲超声通过调节卵母细胞自分泌因子即生长分化因子9(GDF9)和骨形态发生蛋白15(BMP15)的表达在预防环磷酰胺致大鼠卵巢早衰发病中的作用。方法选取63只雌性SD大鼠,其中13只为空白组,其余50只连续5 d腹腔注射环磷酰胺30 mg/kg,建立卵巢早衰动物模型。将造模成功的43只大鼠分为实验对照组(n=21)和治疗组(n=22),治疗组给予低强度脉冲超声辐照,空白组和实验对照组采用与治疗组相同的方法治疗,但仪器无功率输出。Western blotting检测GDF9和BMP15的表达情况。结果最终共纳入大鼠48只,其中空白组11只,实验对照组17只,治疗组20只。实验对照组大鼠腹腔注射环磷酰胺后食欲下降,毛发无光泽,活动差;治疗组大鼠超声辐照后较实验对照组一般情况明显好转,毛色恢复光泽,反应较为灵敏。与空白组比较,实验对照组超声辐照后GDF9和BMP15表达均显著下降(均P=0.00);与实验对照组比较,治疗组超声辐照后GDF9和BMP15表达均显著增强(P=0.01、0.00)。结论低强度脉冲超声能促进大鼠卵巢GDF9和BMP15的表达,从而修复卵巢功能,促进卵泡的发育。

Objective To explore the preventive effect of low intensity pulsed ultrasound on cyclophosphamide induced premature ovarian failure through regulation of expression of oocyte autocrine factor growth differentiation factor 9（GDF9） and bone morphogenetic protein 15（BMP15）.Methods Sixty-three rats were selected. 13 rats were randomly selected as normal group,and the other 50 rats were intraperitoneally injected with cyclophosphamide 30 mg/kg for 5 d to establish the animal model of premature ovarian failure. The successfully established 43 animal model rats were randomly divided into control group（n=21）and treatment group（n=22）.The treatment group was treated with low-intensity pulsed ultrasound,and the normal and control group were treated with the same method as the treatment group,but the instrument had no power output.Western blotting was used to detect the expression of GDF9 and BMP15. Results A total of 48 rats were enrolled in this study,11 rats in normal group,17 rats in control group,and 20 rats in treatment group.In control group,the appetite was decreased,hair was no lustrous and activity was poor in rats after the intraperitoneal injection of cyclophosphamide. Compared with control group,the general situation of the rats in treatment group was improved significantly after ultrasonic irradiation,and the hair was recovered lustrous and the reaction was sensitive. Compared with normal group,the expression of GDF9 and BMP15 decreased significantly after ultrasonic irradiation in control group（both P =0.00）.Compared with control group,the expression of GDF9 and BMP15 increased significantly after ultrasonic irradiation in treatment group（P = 0. 01,0. 00）. Conclusion Low intensity pulsed ultrasound can promote the expression of GDF9 and BMP15,repaire the function of the ovary and promote the follicular development.