电针天枢穴对溃疡性结肠炎大鼠NF-κB信号通路的影响

Effect of Electro-needling ST25 on NF-κB Signaling Pathway in UC Rats

目的:探讨电针天枢穴对溃疡性结肠炎(UC)大鼠NF-κB信号通路的影响。方法:SD大鼠60只随机分为3组:空白组、模型组和电针组,每组各20只,其中模型组和电针组采用TNBS溶液灌肠造模,造模后电针组给予针刺双侧天枢穴电针,空白组和模型组动物每天同一时间放置笼中固定。通过光镜下观察大鼠肠黏膜组织病理学改变,记录分析溃疡性结肠炎结肠黏膜损伤指数(CMDI);酶联免疫吸附法(ELISA法)测定大鼠血清白介素1(IL-1)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α);qRT-PCR法检测肠粘膜NF-κB p65 mRNA的表达。结果:与空白组比较,模型组大鼠溃疡性结肠炎结肠黏膜损伤指数明显升高(P<0.01),血清IL-1、IL-6、TNF-α明显升高(P<0.05),NF-κB p65mRNA的表达明显升高(P<0.05);与模型组比较,电针组大鼠溃疡性结肠炎结肠黏膜损伤指数明显降低(P<0.05),血清IL-1、IL-6、TNF-α明显降低(P<0.05),NF-κB p65 mRNA的表达明显降低(P<0.05)。结论:电针天枢穴可以抑制肠道炎症反应,降低NF-κB的表达,从而达到改善UC炎症状态及免疫应答的作用。

Objective: To investigate the effect of point ST25 with electro-acupuncture on signaling pathway of NF-κB in rats with ulcerative colitis( UC). Methods: 60 SD rats were randomly divided into the blank control group,the model group,and the electro-acupuncture group,with 20 rats in each group. TNBS was used to establish UC model. The electro-acupuncture group was treated with electro-needling on ST25,the rats in the blank control group and the model group were kept in cages at the same time everyday. The changes of intestinal mucosa were observed under light microscope; colon mucosa damage index( CMDI) was analyzed and recorded; levels of IL-1,IL-6,and TNF-α were measured with ELISA method; the mRNA expression of NF-κB p65 was detected with qRT-PCR technique. Results: Compared to the blank control group,CMDI was significantly increased( P < 0. 01),the serum levels of IL-1,IL-6,and TNF-α,as well as mRNA expression of NF-κB p65 were also greatly increased( P < 0. 05) in the model group; they were all significantly decreased in the electro-acupuncture group compared to those in the model group( P < 0. 05). Conclusion: Electro-needling ST25 can relieve the inflammatory reaction of intestine and decrease NF-κB expressions to improve inflammatory state and immune response of UC.