胱抑素C及其基因多态性与广西地区壮族人群冠心病的相关性

Association of cystatin C and its gene polymorphism with coronary heart disease in Guangxi Zhuang population

目的研究胱抑素C(Cys-C)水平及其基因位点+148G/A、+73A/G和-82G/C的多态性与广西壮族冠心病(CHD)的相关性。方法采用免疫比浊方法检测壮族CHD组、汉族CHD组和壮族健康组、汉族健康组血清Cys-C水平;采用聚合酶链反应-限制性片段长度多态性技术检测Cys-C基因位点+148、+73和-82的多态性,并收集各组临床资料。结果与2个健康组比较,2个CHD组临床资料和血清Cys-C水平差异有统计学意义(P<0.05)。4组中外周血Cys-C水平与肌酐(Cr)水平呈正相关(r=0.597,P<0.05)。2个CHD组Cys-C基因+73位点不同基因型分布频率与健康组不同,差异有统计学意义(P<0.05)。Cys-C+73位点GG型CHD患者Cys-C水平明显低于AG型和AA型,差异有统计学意义(P<0.05);AG型与AA型间Cys-C水平比较差异无统计学意义(P>0.05)。结论肾功能受损掩盖Cys-C+73基因多态性所致的Cys-C低水平。Cys-C+73基因多态性所致的Cys-C低水平可能是广西地区壮族、汉族CHD患者的1个危险因素。

Objective To investigate the association between the levels of cystatin C(Cys-C)and its+148 G/A,+73 A/G and-82 G/C loci in Guangxi Zhuang ethnic groups with CHD.Methods The levels of serum Cys-C in CHD group,CHD group,Zhuang healthy group and Han healthy group were detected by immune turbidimetry.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to detect the polymorphisms of+148,+73 and-82 at Cys-C locus,and the clinical data of each group was collected.Results Compared with the two healthy groups,the clinical data and serum Cys-C levels in the two CHD groups were different,the differences were statistically significant(P<0.05).There was a positive correlation between Cys-C levels and creatinine(Cr)levels in the 4 groups(r=0.597,P<0.05).The frequency distribution of Cys-C gene+73 locus in two CHD groups was different from that in healthy group,the differences were statistically significant(P<0.05).The level of Cys-C in patients of CHD with GG type at Cys-C +73 site was lower than those of AG type and AA type,the difference was statistically significant(P<0.05).There was no significant difference in Cys-C level between AG type and AA type(P>0.05).Conclusion Impaired renal function masks Cys-C low levels due to Cys-C+73 gene polymorphisms.The Cys-C low level caused by Cys-C+73 gene polymorphism may be a risk factor for CHD patients in Zhuang and Han ethnic groups in Guangxi.