胃癌患者血浆24种氨基酸代谢物水平变化

Variation of 24 plasma amino acid metabolite levels in patients with gastric cancer

目的研究胃癌患者血浆氨基酸代谢物中是否有可作为胃癌诊断的生物标志物。方法采用超高液相色谱串联质谱(UHPLC-MS/MS)法检测血浆24种氨基酸水平,比较胃癌患者与正常人群、胃癌患者不同分期、胃癌患者术前与术后的变化。结果胃癌患者血浆中丙氨酸(Ala)、甘氨酸(Gly)、谷氨酸(Glu)等18种氨基酸水平均较正常对照组下降,缬氨酸(Val)、精氨酸(Arg)、丝氨酸(Ser)水平较正常对照组升高,差异均有统计学意义(P均<0.05),对称性二甲基精氨酸(SDMA)、犬尿氨酸(Kyn)、马尿酸(HA)水平与正常对照组比较差异均无统计学意义(P均>0.05)。Ⅲ~Ⅳ期胃癌患者血浆中的Arg水平高于Ⅰ~Ⅱ期胃癌患者,谷氨酰胺(Gln)、Glu、甲硫氨酸(Met)、苯丙氨酸(Phe)水平低于Ⅰ~Ⅱ期胃癌患者,差异有统计学意义(P均<0.05)。18例胃癌患者术后血浆中亮氨酸(Leu)、Arg、瓜氨酸(Cit)水平分别较术前下降,Gln、赖氨酸(Lys)、Glu、Phe水平较术前升高,差异有统计学意义(P均<0.05)。结论胃癌患者血浆氨基酸代谢物如Gln、Arg在胃癌早期预测中有重要意义。

Objective To study whether plasma amino acid metabolites in patients with gastric cancer can be used as biomarkers for the diagnosis of gastric cancer. Methods The levels of 24 kinds of plasma amino acids were detected by ultra-high-performance liquid chromatography tandem mass spectrometry（UHPLC-MS/MS）, and were compared between patients with gastric cancer and normal controls, between patients with different stages of gastric cancer, and between gastric cancer patients before and after operation. Results The levels of 18 kinds of plasma amino acids including alanine（Ala）, glycine（Gly） and glutamic acid（Glu） in gastric cancer patients were significantly lower than those in the normal controls, while the levels of valine（Val）, arginine（Arg） and serine（Ser） were significantly higher than those in the normal controls（all P〈0.05）; there were no significant differences in symmetric dimethylarginine（SDMA）, kynurenine（Kyn） or hippuric acid（HA） levels between the two groups（P〈0.05）. The plasma level of Arg in patients with Ⅲ-Ⅳstage of gastric cancer was significantly higher than that in theⅠ-Ⅱstage, while the plasma glutamine（Gln）, Glu, methionine（Met） and phenylalanine（Phe） levels were significantly lower than those in theⅠ-Ⅱstage（all P〈0.05）. The levels of plasma leucine（Leu）, Arg and citrulline（Cit） in patients after operation were significantly lower than those before operation, while the plasma Gln, lysine（Lys）, Glu and Phe levels were significantly higher than those before operation（all P〈0.05）. Conclusion Amino acids metabolites in plasma of patients with gastric cancer such as Gln and Arg play important roles in the early prediction of gastric cancer.