UPLC-MS/MS MRM模式同时测定参白方中人参皂苷Re、人参皂苷Rg1、人参皂苷Rb1以及Siderin 4种成分含量

Simultaneous Determination of Ginsenoside Re,Ginsenoside Rg1,Ginsenoside Rb1 and Siderin in Shenbai Formula by UPLC-MS/MS MRM

目的:建立参白方中人参皂苷Re、人参皂苷Rg1、Siderin以及人参皂苷Rb1 UPLC-MS/MS含量测定方法。方法:采用Waters ACQUITY UPLCTM HSS T3(2.1 mm×100 mm,1.8μm)色谱柱;流动相:A为0.1%甲酸甲醇,B为0.1%甲酸水,梯度洗脱。采用电喷雾电离源(ESI),多反应离子监测(MRM)扫描模式。结果:参白方中被检测的4种成分线性关系良好;精密度,重复性,稳定性良好;加样回收率在99.86%~100.97%,RSD 0.91%~2.74%。结论:该方法简单、准确、灵敏、重复性好,可用于参白方主要成分的含量测定。

Objective: To establish an UPLC-MS/MS method for the determination of ginsenoside Re,ginsenoside Rg1,siderin and ginsenoside Rb1 in Shenbai formula. Methods: Waters ACQUITY UPLCTM HSS T3 column(2. 1 mm × 100 mm,1. 8 μm) was adopted; mobile phase: 0. 1% formic acid/methanol and 0. 1% formic acid/water in a gradient mode; electrospray ionization(ESI) and multi reaction ion monitoring(MRM) scanning were applied. Results: Four compounds showed a good linear relationship with good precision,repeatability and stability; the recovery rate was99. 86% ~ 100. 97%,and RSD was 0. 91% ~ 2. 74%. Conclusion: This method is simple,accurate,sensitive and reproducible. It can be used for the content determination of the main constituents in Shenbai formula.