气质质联用法测定水产品中50种农药残留量

Determination of 50 Pesticide Residues in Aquatic Products by Gas Chromatography/Mass Spectrometry/Mass Spectrometry

建立气相色谱/串联三重四级杆质谱同时测定鲑鱼、对虾中50种农药残留的方法。对三重四级杆质谱(MS/MS)检测参数及样品前处理方式进行优化。样品经乙腈提取后,冷冻、离心,Qu ECh ERS方法净化,采用气相色谱/串联三重四级杆质谱仪检测,50种农药在线性范围内均呈现良好的线性关系,线性系数大于0.99。本检测方法的检出限(LOD)为0.001 mg/kg^0.005 mg/kg,方法验证试验结果表明,该类化合物的平均回收率在65%~118%之间,相对标准偏差(RSD)在0.74%~12.3%之间。本方法重现性好,精密度高,操作简单,适用于鲑鱼、对虾等水产品中多种农药残留的检测。

A multiresidue analytical method for 50 pesticide residues in salmon and prawn were established by gas chromatography/triple quadrupole mass spectrometry（G C / M S / M S）.The triple quadrupole mass spectrometry （MS/MS） detection parameters and sample pretreatment method were optimized. The samples were extracted by acetonitrile, freezing, centrifuged, and purified by QuEChERS and detected by gas chromatography/ triple quadrupole mass spectrometry （G C / M S / M S ）. The 50 pesticides in linear range were shown good linear rela-tionship , and the linear coefficient was higher than 0.99.The detection limit of this test method （ L O D ） was 0.001 mg/ kg-0.005 mg/kg, and the experimental results of methods verified showed that the recoveries were be-tween 65 〈-118 % and relative standard deviations （RSD） were between 0.74 % - 1 2 . 3 %. The method has good reproducibility, high precision, simple operation, therefore, it is suitable for the detection of a variety of pesti-cide residues in salmon, prawn and other aquatic products.