摘要
试验以鸡血作为发酵原料,研究复合菌种扩大培养条件,并利用响应面对其进行优化。以温度、pH值、转速为影响因子,菌液浓度的OD值作为指标的单因素的试验基础上,根据中心组合Box Behnken(BBD)的设计原理设计出三因素三水平的试验,得到最佳的混合菌株的发酵条件为:温度30℃、pH=7、转速140 r/min,在此优化的条件下OD值达到最大。为进一步对动物副产品的研究和开发提供了一定的科研和应用价值。
The chicken blood as the fermented materials, the expanding culture conditions for composite strains was studied and optimized by response surface . The temperature, pH and rotational speed were as the factors, based on the OD value of bacteria concentration as the single factor index. According to the design principle of Box Behnken （BBD） in the center combination, three factors and three levels experiments were designed to obtain the optimum fermented conditions for the mixed strains： temperature 30 ℃, pH=7, rotational speed 140 r/min, under these optimal conditions, the OD value reached the maximum. This experiment provides scientific research and application value for further research and development of animal by-products.
出处
《食品研究与开发》
CAS
北大核心
2018年第3期195-201,共7页
Food Research and Development
关键词
鸡血
发酵
菌株
生长曲线
响应面分析法
chicken blood
fermentation
strain
growth curve
response surface methodology：
