假单胞菌HC5对马铃薯晚疫病菌的抑制作用研究

Study on Antagonistic Activity on P.infestans Exhibited from Pseudomonas sp.HC5

从土壤中分离获得对马铃薯晚疫病菌具有较强拮抗作用的假单胞菌HC5。采用皿内涂布法、平板对峙法和平板渗透法分别评价了HC5菌株发酵液对马铃薯晚疫病菌的抑制能力,采用平板渗透法进一步研究了菌株HC5发酵液在不同温度、p H以及紫外线照射时间下对马铃薯晚疫病菌抑制的稳定性。结果显示,不同方法下,菌株HC5对马铃薯晚疫病菌均具有较强的抑制作用,与对照组相比,病原菌几乎不生长。菌株HC5发酵液在温度50℃以下,pH为5~11时,紫外线照射120 min均对马铃薯晚疫病菌具有抑制作用,即菌株HC5的耐受稳定性较强。

Pseudomonas sp.HC5 which have strong antagonistic efficacy on P.infestans was isolated from the soil.The antagonistic ability of strain HC5 fermentation liquor on P.infestans were evaluated with surface spread plate mothed,plate confront antibiotic mothed and plate penetration mothed.With plate penetration mothed,the stabilities of strain HC5 fermentation liquor under different temperature treatments,different p H treatments and different time of ultraviolet light treatments were studied.The results show that strain HC5 all have strong antagonistic efficacy on P.infestans,and pathogen did not grow comparing to the control.The strain HC5 fermentation liquor have antagonistic efficacy on P.infestans below temperature 50 ℃,between pH 5~11 and 120 minutes in ultraviolet light treatment.The strain HC5 have strong tolerance and stability.