醋酸钠及复配4种矿物质对草菇菌丝生物量的影响

Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea

通过测定菌丝DNA含量,检测不同浓度梯度醋酸钠对草菇菌丝生物量的影响,结果表明,随着醋酸钠浓度的升高,菌丝生物量呈线性增加,当达到0.7 g·L^(-1)时,生物量达到一个高峰值,DNA的含量为468.9±4.10μg·g^(-1),极显著地高于对照组,随着醋酸钠浓度继续增加到0.9 g·L^(-1)和1.1 g·L^(-1)时,生物量则呈减少的趋势,但均比对照的生物量高,说明适宜的醋酸钠浓度能极显著地增加草菇菌丝生物量。在筛选出最佳醋酸钠浓度(0.7 g·L^(-1))的基础上,进一步复配Cu、Zn、K、Mn元素,以醋酸钠为对照,比较复配微量元素及同一微量元素不同浓度对草菇菌丝生物量的影响。结果显示,添加矿物质K和Cu元素效果较好,各浓度均能使菌丝生物量有所增加,其中添加8 g·L^(-1)KH_2PO_4处理的菌丝生物量比对照的高84.32%(P<0.05);添加0.04 g·L^(-1)Cu SO_4处理的菌丝生物量比对照高73.19%,达到5%显著水平;Zn SO_4添加量为0.25 g·L^(-1)和0.20 g·L^(-1)两处理的菌丝生物量分别比对照提高42.69%和20.34%;添加0.35 g·L^(-1)Mn SO_4处理的菌丝生物量比对照提高25.23%以上。Zn和Mn元素的各浓度处理与对照的菌丝体生物量无显著差异。

In this paper,mycelial dry weight of mycelium and DNA content of Volvariella volvacea were determined to detect the effect of different concentrations of sodium acetate on mycelial biomass of V. volvacea. The results showed that with the increase of concentration of sodium acetate,mycelial biomass increased linearly. When the concentration reached 0. 7 g·L^(-1),the biomass reached the peak,and the content of DNA was 468. 9 ± 4. 10 μg·g^(-1),which was significantly higher than that of CK. While the concentration of sodium acetate was 0. 9 g·L^(-1) and 1. 1 g·L^(-1),the biomass decreased,but higher than that of CK. It was concluded that the suitable concentration of sodium acetate can significantly increase the mycelium dry weight of V. volvacea. Based on the optimal concentration of sodium acetate( 0. 7 g·L^(-1)),the effect of Cu,Zn,K,Mn element and compound trace element on mycelia biomasswere compared. The results showed that K and Cu element showed good effect,the mycelial biomass under all concentrations increased. Compared with CK,when the application amount of KH_2 PO_4 was 8 g·L^(-1),the mycelial biomass was significantly increased by 84. 32%( P < 0. 05); when the application amount of Cu SO_4 was 0. 04 g·L^(-1),the mycelia biomass was significantly increased by 73. 19%( P < 0. 05); when the application amount of Zn SO_4 were0. 25 g·L^(-1) and 0. 20 g·L^(-1),the mycelia biomass was increased by 42. 69% and 20. 34%,respectively; when the application amount of Mn SO_4 was 0. 35 g·L^(-1),the mycelia biomass was increased by 25. 23%. The Zn element and Mn element showed no significant effect on mycelium biomass with CK.