基于PCR-RFLP技术分析H-FABP基因在5个猪群体中的多态性

Analysis of H-FABP Gene Polymorphisms in Five Pig Groups Based on PCR-RFLP

【目的】肉质是猪肉的重要经济指标,肌内脂肪(IMF)含量是影响肉质的重要因素。H-FABP基因是调控猪肉IMF含量的候选基因。【方法】本实验采用PCR-RFLP技术分析H-FABP基因多态性,并检测该基因在5个猪群体:杜洛克(100头)、长白(80头)、大白(150头)、申农(80头)、梅山(140头)的基因型和基因频率。【结果】H-FABP基因在杜洛克、大白、申农猪群体中检测出DD、Dd和dd 3种基因型。在杜洛克群体中的基因型频率:DD(0.02)、Dd(0.23)和dd(0.75);在大白猪群体中基因型频率DD∶(0.25)、Dd(0.49)和dd(0.26);在申农猪群体中基因型频率:DD(0.10)、Dd(0.30)和dd(0.60)。在长白猪群体中检测的所有样品为dd型;在梅山猪群体中检测的所有样品为DD型。【结论】H-FABP基因在5个猪群体中呈现不同基因型。在长白猪和梅山猪群体中呈现偏态现象。在杜洛克和申农猪群体中主要为dd型,在大白群体中主要为Dd型。

【Objective】Meat quality is one of the important indicators of pork. Intramuscular fat content( IMF) is one of the important factors in affecting meat quality. H-FABP gene is a candidate gene as regulating pig intramuscular fat traits. 【Method】The experiment based on PCR-RFLP was conducted,and H-FABP gene polymorphisms in five pig groups: Duroc( 100),Landrace( 80),Large White( 150),Shennong( 80) and Meishan( 140) were detected. 【Result】H-FABP genes in Duroc,Large White and Shennong groups had DD,Dd and dd genotype. The genotype frequencies in Duroc groups were DD( 0. 02),Dd( 0. 23) and dd( 0. 75),the genotype frequencies in Large White groups were DD( 0. 25),Dd( 0. 49) and dd( 0. 26),and the genotype frequencies in Shennong groups were DD( 0. 10),Dd( 0. 30)and dd( 0. 60). The genotype frequencies in Landrace groups were dd( 1. 0),no Dd and DD; Genotype frequencies in Meishan groups: DD( 1. 0). 【Conclusion】H-FABP gene present different genotypes in five pig groups. Only gentype was detected in Landrace and Meishan populations. dd genotype was main genotype in Duroc and shennong groups,Dd genotype was main genotype in Large White groups.