不同蛋白酶水解鹿鞭制备活性肽的比较研究

Comparative Study on Preparation of Active Peptides Hydrolyze by Different Proteases

[目的]研究制备鹿鞭活性肽的最佳蛋白酶。[方法]选用碱性蛋白酶、中性蛋白酶、复合蛋白酶和胰蛋白酶分别在最适条件下水解鹿鞭,以水解物鹿鞭活性肽的水解度(DH)及其对还原能力、抑制硫代巴比妥酸(TBARS)的作用和清除DPPH自由基作用的影响为指标,优选出最佳水解蛋白酶。[结果]碱性蛋白酶水解鹿鞭产物鹿鞭活性肽的水解度、还原能力、对卵磷脂脂质氧化的抑制作用和DPPH自由基的清除率都为最高。[结论]碱性蛋白酶水解鹿鞭产物鹿鞭活性肽具有很好的抗氧化性。

[ Objective]The research aimed to study the optimal protease for preparation of deer penis active peptide. [ Method] The alkaline protease , neutral protease, compound protease and trypsin were respectively used to hydrolyze deer penis under optimum conditions, the degree of hydrolysis （DH） of the hydrolyzate deer penis active peptide and its effect on reduction, inhibition of TBARS and scavenging of DPPH free radical were taken as indexes to optimize the optimal hydrolytic protease. [ Result] The degree of hydrolysis, reducing ability, inhibition of leci-thin lipid oxidation and DPPH radical scavenging rate of alkaline protease hydrolysis deer penis active peptide were the highest. [ Method ] Al-kaline protease hydrolysis deer penis product deer penis activite peptide has good oxidation resistance.