基于近红外量子点的荧光共振能量转移生物探针构建及应用

Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots

本论文构建了基于近红外量子点In P/Zn S和Cy7(C45H44K3N3O16S4)的荧光共振能量转移(FRET)体系,完成了不同p H值和不同浓度下的FRET体系转换效率的检测。检测结果显示:当量子点浓度保持不变时,随着染料浓度的增加,体系转换效率也随之增加,当In P/Zn S量子点与Cy7浓度比为1∶250时,转换效率高达68%。细胞测试结果表明,FRET体系对p H值有较高敏感度,对细胞微环境p H值的检测精度可达0.1,该体系可以作为敏感型FRET探针用于生物微环境检测。

In this paper,a kind of fluorescence resonance energy transfer( FRET) system based on near infrared In P/Zn S Quantum Dots and fluorescence dye Cy7 was constructed,and the conversion efficiencies of FRET system at different p H values and different concentrations were measured. Experimental results indicatedthat when the concentration of quanium dots remained constant,the conversion efficiency of the system increased with the increasing of the concentration of dye. When the concentration ratio of In P/Zn S and Cy7 was1 ∶250,the conversion efficiency was 68%. The results of cell test showed that the FRET system had a high sensitivity to p H value,and the detection accuracy of p H value for cell microenvironment was 0. 1,which could be used as a sensitive FRET probe for biological microenvironment detection.