应用间接免疫荧光法诊断鸭短嘴矮小综合征

Diagnosis of Duckling Short Beak and Dwarfism Syndrome by Indirect Immunofluorescence Assay

应用抗鹅细小病毒(GPV)单克隆抗体(Mab E16)为一抗,建立检测鸭短嘴矮小综合征病毒(SBDSGPV)的间接免疫荧光方法 (IFA),并对人工感染SBDS-GPV的发病鸭和临床送检鸭短嘴矮小综合征(SBDS)病鸭的肝脏、脾脏、肾脏和胰腺进行检测。结果显示该方法具有良好的特异性,能识别鹅细小病毒抗原呈现亮绿色特异荧光,而与MPV、MDRV、NDRV、DHV、DPV、PMV等其他鸭病病原均无交叉反应;应用该方法检测SBDS-GPV人工感染发病鸭和SBDS临床病鸭组织,均不同程度出现亮绿色荧光,其中人工感染发病鸭阳性率100%(5/5),临床送检病鸭阳性率91.7%(11/12)。表明建立的IFA具有快速、特异等优点,可用于SBDS的快速诊断。

An indirect immunofluorescence assay(IFA)was established using the monoclonal antibodies(Mab E16)against goose parvovirus(GPV)for identification of the short beak and dwarfism syndrome(SBDS)-GPV in ducks.Liver,spleen,kidney and pancreas of the ducklings challenged with SBDS-GPV and the natural infection cases were tested by the new IFA methodology.The results showed that the newly developed IFA was specific,giving positive reaction to GPV only and negative to other viruses of MPV,MDRV,NDRV,DHV,DPV and PMV.Five out of 5challenged cases and 11 out of 12 natural infection cases showed a positive reaction with bright green fluorescence glow on IFA.Consequently,the new method could be applicable for detecting and diagnosing SBDS in ducks with high specificity,accuracy and rapidity.