摘要
[目的]探明刚地弓形虫在伊宁市宠物犬猫、流浪犬、散养猫中的感染情况及流行特点。[方法]分别采用动物弓形虫Ig G抗体检测试剂盒(酶联免疫吸附试验)及弓形虫抗体快速诊断试纸(免疫色谱分析法)对不同生活状态、不同年龄、不同季节的440份犬猫血清样品进行抗体检测。[结果]酶联免疫吸附试验检测阳性率为20.23%(89/440),宠物犬阳性率为15.50%(31/200),宠物猫阳性率为10.00%(2/20),流浪犬阳性率为24.00%(48/200),流浪猫阳性率为40.00%(8/20),流浪犬阳性率显著高于宠物犬(P<0.05),散养猫阳性率显著高于宠物猫(P<0.05);第二季度犬的感染率最高,为27.00%(27/100),与第一季度差异显著(P<0.05);1岁以内犬组与6岁以上犬组差异极显著(P<0.01),1~3岁与6岁以上犬组差异显著(P<0.05);弓形虫抗体快速诊断试纸(免疫色谱分析法)检测阳性率为21.14%,与酶联免疫吸附试验检测方法差异不显著(P>0.05)。[结论]伊宁市犬猫弓形虫抗体阳性率处于较高水平,阳性率高低与犬猫年龄、生活状态以及季节性等因素有关。
[Objective]To find out the infection status and epidemic characteristics of toxoplasma gondii in the stray cats of pet dogs and cats in Yining City.[Method]440 dogs and cats antibody serum samples with different living conditions,different ages and different seasons were tested by animals toxoplasma Ig G antibody detection kit( enzyme-linked immunosorbent assay) and toxoplasma antibody rapid diagnostic test paper( Immunochromatographic analysis). [Result] The positive rate of enzyme-linked immunosorbent assay was 20. 23%( 89/440),pet dogs were15. 50%( 31/200),pet cats were 10. 00%( 2/20),stray dogs were 24. 00%( 48/200),stray cats were 40. 00%( 8/20). The positive rate of stray dogs was significantly higher than that of pet dogs( P < 0. 05),the positive rate of free range cat was significantly higher than that of pet cat( P < 0. 05). The infection rate of dogs in the second quarter was the highest,at 27. 00%( 27/100),which was significantly different from the first quarter( P < 0. 05). The difference between less than 1 year old group and more than 6 years old group was highly significant( P < 0. 01).Difference between 1-3 years old group and over 6 years old group was significant( P < 0. 05). Immuno chromatographic analysis was used to detect the positive rate of 21. 14%,which was not significant( P > 0. 05).[Conclusion]The positive rate of toxoplasma gondii was at a higher level in Yining City. The rate of positive rate was related to the age,state of life and seasonality of dogs and cats.
出处
《安徽农业科学》
CAS
2018年第3期71-73,共3页
Journal of Anhui Agricultural Sciences
关键词
伊宁市
犬
猫
弓形虫
流行病学
Yining City
Dog
Cat
Toxoplasmosis
Epidemiology