2株海洋红酵母突变菌株对小鼠的毒性试验

Toxicity Test of Two Mutation Strains of Marine Rhodotorula on Mice

[目的]研究2株海洋红酵母突变菌株对小鼠的急性毒性。[方法]将42只小鼠随机分为7组,试验组(1)、(2)和(3)小鼠灌服胶红酵母J6-82菌悬液,试验组(4)、(5)和(6)小鼠灌服粘红酵母J2-75菌悬液,细菌数量分别为1×107、1×109和1×1011个/m L,对照组小鼠灌服灭菌的红酵母培养基,研究胶红酵母J6-82和粘红酵母J2-75对小鼠的急性毒性。[结果]42只小鼠临床健康,无发病和死亡,无病理解剖学变化;试验组小鼠的平均体质量、饮水量、脏器指数与对照组相比无显著差异,淋巴细胞总数、淋巴细胞比率与对照组相比差异显著;试验组小鼠肝脏组织切片无病理组织学变化。[结论]胶红酵母J6-82和粘红酵母J2-75对小鼠无急性毒性。

[Objective] To study the acute toxicity of two mutation strains of marine Rhodotorula on mice.[Method]42 mice were randomly divided into 7 groups. Mice in test group(1),(2),(3) were fed with Rhodotorula mucilaginosa J6-82 bacteria suspension with the bacteria number of1 × 107,1 ×109 and 1 × 1011 ind./m L respectively. Mice in testl group(4),(5),(6) were fed with Rhodotorula glutinis J2-75 with bacteria number of 1 × 107,1 ×109,1 ×1011 ind./m L respectively. The mice in control group were fed with sterilized red yeast culture medium. The acute toxicity of R. mucilaginosa J6-82 and R. glutinis J2-75 on mice were studied.[Result]42 mice were clinical healthy,there was no incidence of death or pathological changes. There was no significant difference between the control group and test groups in average body mass,drinking water amount,organ index of mice,but total lymphocyte count and lymphocyte ratio had significant differences between test groups and control group.There was no histopathological change in liver tissue of mice in test groups.[Conclusion]Rhodotorula mucilaginosa J6-82 and Rhodotorula glutinis J2-75 had no acute toxicity on mice.