热量限制及隔日禁食对小鼠氧化应激水平的影响

Effects of Calorie Restriction and Alternate Day Fasting on Oxidative Stress in Mice

目的观察热量限制和隔日禁食对12月龄雄性c57小鼠SOD、GSH-Px、MDA水平的影响。方法 12月龄30只雄性c57小鼠随机分为3组:对照组、热量限制组、隔日禁食组,经过正常喂养,限制能量摄入(给予正常饮食量的60%)和隔日禁食16周,建立热量限制和隔日禁食动物模型。取对照组、热量限制组及隔日禁食组的肝脏,测定SOD活性、GSH-Px活性和MDA含量。结果与对照组相比,热量限制组SOD和GSH活性显著增高(P<0.05),MDA显著降低(P<0.05);与对照组相比,隔日禁食组SOD和GSH活性增高(P<0.05),MDA略有降低(P>0.05);与隔日禁食组相比,热量限制组SOD和GSH活性都略有增高,MDA略有降低(P>0.05)。结论热量限制或隔日禁食均可在不同程度上改善小鼠的氧化应激水平,但热量限制较隔日禁食对小鼠氧化应激水平的影响无明显区别。

Objective To observe the effect o f caloric restriction and alternate day fasting on the levels o f SOD, GSH- Px and MDA in 12-month-old male c57 mice. Methods 32 male c57 mice of 12 months old were randomly divided into 3 groups： control group （normal feeding）, caloric restriction group （fed with 60% of normal diet）, alternate day fasting group; after 16 weeks, animal models were established; the livers of the mice in each group were taken to measure SOD activity, GSH activity and MDA content. Results The activities of SOD and GSH in caloric restriction group increased much higher while MDA dropped down lower than those in control group （^〈0.05）; the activities of SOD and GSH in alternate day fasting group increased higher while MDA dropped down slightly lower than those in control group （P〈0.05）; the activities of SOD and GSH in caloric restriction group increased slightly higher while MDA dropped down slightly lower than those in alternate day fasting group （P〈 0.05）. Conclusion To varying degrees, caloric restriction and alternate day fasting can improve the oxidative stress in mice at certain degree, caloric restriction is more effective in improving the oxidative stress balance in mice than alternate day fasting.