摘要
目的:探索心肌梗死相关微小RNA(microRNA,miR)-678的功能及其调控的下游靶基因。方法:利用H2C9心肌细胞建立缺血缺氧模型,研究H2C9细胞氧糖剥夺后miR-678的表达;利用细胞转染技术过表达miR-678,检测H2C9细胞增殖情况;在microRNA数据库中,寻找miR-678可能结合的下游靶基因的3'非编码序列,并利用细胞转染技术在H2C9细胞中导入外源性miR-678模拟物或者抑制剂,验证miR-678对靶基因的调控。结果:miR-678可促进H2C9细胞增殖,在心肌细胞缺血后下调;数据库检索发现miR-678序列能配对FoxP1基因mRNA的3'序列;在H2C9细胞中导入外源性miR-678模拟物能下调FoxP1蛋白,而导入外源性miR-678抑制剂能上调FoxP1蛋白。结论:缺血心肌细胞miR-678的表达水平下调,miR-678在心肌细胞中可能的下游靶点为FoxP1蛋白。
Objective:To study the function of miR-678 related to myocardial infarction(MI) and the downstream target genes regulated by it.Methods:We investigated the expression of miR-678 after deprivation of oxygen and glucose,using the ischemic model of H2C9 cardiomyocytes in vitro.After overexpression of miR-678 by using transfection technology,we determined the proliferation of H2C9 cells.To explore the possible 3 ' non-coding sequences of downstream target genes binding by miR-678,we scanned the online database of microRNAs.We transfected exogenous mimic or inhibitor of miR-678 into H2C9 cells to validate the target gene regulated by miR-678.Results:MiR-678 could promot the proliferation of H2C9 cells and was down-regulated after myocardial ischemia.We searched the database and found that the 3 'sequences of FoxP1 mRNAcould clearly match with miR-678.Transfection of exogenous miR-678 mimic or inhibitor into H2C9 cells could down-regulate or up-regulate FoxP1 protein.Conclusions:The expression of miR-678 in ischemic cardiomyocyte is down-regulated,and the possible downstream target of miR-678 in cardiomyocyte is FoxP1 protein.
出处
《国际心血管病杂志》
2018年第1期44-47,共4页
International Journal of Cardiovascular Disease
基金
上海市卫生和计划生育委员会(20134376)
