坦洋工夫红茶多糖提取工艺优化及其抑制肿瘤活性分析

Optimization of Extraction and Antitumor Activity of Polysaccharide from Tanyang Congou Black Tea

以坦洋工夫红茶为原料,以多糖得率为考察指标,在单因素试验的基础上,采用响应面分析法优化红茶多糖提取工艺,其最佳工艺条件为液料比30∶1(m L/g)、提取温度60℃、提取时间70 min,多糖得率达10.52%。经纯化后多糖质量分数达76%,含有糖醛酸和吡喃环结构,主要由阿拉伯糖(34%)、半乳糖醛酸(30%)、葡萄糖(14%)、半乳糖(13%)、岩藻糖(5%)和鼠李糖(4%)组成。BALB/c小鼠体内抑制肿瘤实验表明,红茶多糖可显著抑制荷瘤小鼠体内实体肿瘤的生长,并有效保护其脾脏和胸腺。该实验结果为红茶多糖的提取及其抗肿瘤活性研究提供一定理论依据。

Optimization of the extraction of polysaccharide from Tanyang Congou black tea for higher polysaccharide yield was carried out using one-factor-at-a-time method and response surface methodology. The optimal conditions that provided maximum polysaccharide yield（10.52%） were found as follows： temperature, 60 ℃; extraction time, 70 min; and solvent-tosolid ratio, 30：1（m L/g）. The purified polysaccharide（76% purity） contained uronic acid and pyranose rings, and consisted of arabinose（34%）, galacturonic acid（30%）, glucose（14%）, galactose（13%）, fucose（5%）, and rhamnose（4%）. Furthermore, the polysaccharide showed a remarkable inhibitory effect on the growth of solid tumors in H22-bearing BALB/c mice and protected spleen and thymus against tumor damage. These results provide an experimental basis for the extraction and antitumor activity evaluation of polysaccharide from black tea.