响应面法优化金蝉花多糖提取工艺及抗氧化活性分析

Optimization of Extraction and Antioxidant Activity of Polysaccharides from Cordyceps cicadae by Response Surface Methodology

通过考察液料比、浸提时间及浸提温度对金蝉花多糖含量的影响,在单因素试验基础上进行响应面优化提取工艺条件,并通过测定金蝉花多糖总还原力、清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)自由基、羟自由基(·OH)和超氧阴离子自由基(O_2^-·)的能力研究其体外抗氧化活性。结果表明,金蝉花多糖适宜的提取工艺参数为浸提时间130?min、浸提温度80℃、液料比50∶1(m L/g),在此条件下金蝉花多糖含量实际值为26.14?mg/g。金蝉花多糖具有较好的抗氧化能力,其清除DPPH自由基、·OH、O_2^-·的半抑制质量浓度(IC_(50))分别为28.99?μg/m L、0.19?mg/m L和0.30?mg/m L。

The extraction of water-soluble polysaccharides（WSP） from Cordyceps cicadae was optimized using one-factorat-a-time method and response surface methodology. Solvent-to-solid ratio, extraction time and temperature were used as independent variables while polysaccharide yield was taken as response variable. The in vitro antioxidant activity of WSP was investigated by measuring total reducing power and scavenging activities against 1,1-diphenyl-2-trinitrophenylhydrazine（DPPH）, hydroxyl and superoxide anion radicals. The optimum extraction conditions were found to be extraction at 80 ℃ for 130 min with a solvent-to-solid ratio of 50：1（m L/g）. Experiments conducted under these conditions gave a polysaccharide yield of 26.14 mg/g. WSP possessed strong antioxidant activity with half inhibitory concentration（IC50） for scavenging of DPPH, hydroxyl and superoxide anion radicals 28.99 μg/m L, 0.19 mg/m L and 0.30 mg/m L, respectively.