硫化氢通过调节Sirt1/eNOS信号通路延缓人脐静脉内皮细胞衰老

Hydrogen sulfide attenuates human umbilical vein endothelial cell senescence via modulation of Sirt1/eNOS pathway

目的:我们既往研究已证实外源性硫化氢能够延缓内皮细胞衰老,本研究拟进一步探讨沉默信息调节因子1(Sirt1)与内皮型一氧化氮合酶(e NOS)系统对硫化氢抗内皮细胞衰老作用的影响。方法:建立葡萄糖(33 mmol/L)诱导的人脐静脉内皮细胞(HUVECs)衰老模型,根据细胞活力、衰老相关β-半乳糖苷酶(SA-β-Gal)染色阳性率和纤溶酶原激活物抑制剂1(PAI-1)的表达水平评估HUVECs衰老;同时采用RNA干扰技术抑制Sirt1蛋白表达,检测e NOS表达及衰老相关指标。结果:HUVECs经高糖处理后,细胞活力减低,SA-β-Gal阳性细胞比例增加,PAI-1表达增高,Sirt1及e NOS表达均明显减少(P<0.05);与高糖处理组比较,100μmol/L硫氢化钠(硫化氢供体)处理组的细胞活性增加,SA-β-Gal染色阳性细胞比例及PAI-1蛋白表达显著下降,Sirt1及e NOS蛋白表达增加,NO含量增加(P<0.05)。与硫化氢处理组比较,Sirt1 siRNA处理后e NOS表达减少,PAI-1表达增加,SA-β-Gal染色阳性细胞数目增多,NO含量减少(P<0.05或P<0.01)。结论:硫化氢通过上调Sirt1、增加e NOS表达而促进NO的合成,从而抵抗高糖诱导的HUVECs衰老。

AIM： To explore the role of Sirt1/e NOS signalling pathway in the protective effect of hydrogen sulphide（ H2 S） against endothelial cell senescence induced by high glucose. METHODS： High glucose（ 33 mmol/L） was applied to induce senescence in primary human umbilical vein endothelial cells（ HUVECs）. The cell viability,the proportion of senescence-associated β-galactosidase（ SA-β-Gal） positive cells and the plasminogen activator inhibitor 1（ PAI-1）expression were detected to assess the senescence model. Mean while,Sirt1 siRNA was used to examine the effect of Sirt1 on e NOS expression and the senescence-related parameters. RESULTS： Treatment of HUVECs with high glucose decreased the cell viability slowly with a larger proportion of the cells stained with SA-β-Gal,and the protein expression of PAI-1 was dramatically increased. The increased cell viability,reduced SA-β-Gal positive cells and decreased protein expression of PAI-1 were detected after sodium hydrosulfide（ Na HS,100 μmol/L） treatment. Furthermore,Na HS treatment upregulated the protein expression of Sirt1 and e NOS,and eventually increased the production of nitric oxide（ NO）. CONCLUSION： Exogenous H2 S modulates Sirt1/e NOS/NO pathway to prevent HUVECs against high glucose-induced senescence.