1,25二羟基维生素D_3对2型糖尿病大鼠肝脏中维生素D受体、脂联素受体2、过氧化物酶体增殖物激活受体αmRNA表达及甘油三酯含量的影响

Effects of 1,25(OH)_2D_3 on the expression of VDR,AdipoR2,PPAR-αmRNA and triglyceride contents in the liver of type 2 diabetic rats

目的探讨1,25二羟基维生素D_3[1,25(OH)_2D_3]对T2DM大鼠肝脏的保护作用及机制。方法 100只雄性Wistar大鼠随机分成单纯T2DM组、1,25(OH)_2D_3干预组[1,25(OH)_2D_3+DM]、过氧化物酶体增殖物激活受体α(PPAR-α)抑制组[1,25(OH)_2D_3+PPAR-α抑制剂+DM]和正常对照(NC)组,每组各25只。6周后,测定各组FPG及血脂。ELISA检测肝脏组织中TG含量,RT-PCR检测大鼠肝脏组织中维生素D(VitD)受体(VDR)、PPAR-α、脂联素受体2(AdipoR2)mRNA表达水平。结果 1,25(OH)_2D_3+DM组肝脏中VDR、PPAR-α、AdipoR2 mRNA表达较T2DM组增加(t=20.32,5.92,9.10,P<0.05),TG含量较T2DM组减少(t=13.84,P<0.05)。结论 1,25(OH)_2D_3可能通过上调VDR基因的表达,进而上调AdipoR2、PPAR-α基因表达,从而减少肝脏TG的含量。

Objective To explore the protective effect and mechanism of 1,25dihydroxyvitamin D_3[1,25（OH）2D3]on the liver of rats with type 2 diabetes（T2DM）.Methods 100 male Wistar rats were randomly divided into four groups：T2DM group,Vitamin D（VitD）intervention group（VitD＋DM group）,peroxisome proliferator-activated receptors-alpha（PPAR-α）inhibitor group（VitD＋DM＋PPAR-αinhibitor group）and normal control group（NC group）,with 25in each group.After 6 weeks later,fasting serum glucose（FPG）and blood lipid were measured.The content of triglyceride（TG）in liver tissue was detected by enzyme method.The expressions of Vitamin D receptor（VDR）,PPAR-αand adiponectin receptor 2（AdipoR2）mRNA in rat liver was detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction（RT-PCR）.Results The expressions of VDR,PPAR-αand Adipo R2 mRNA were significantly increased in VitD intervention group compared with T2DM group（t=20.32,5.92,9.10;P〈0.05）.The content of TG in VitD＋T2DM group was significantly lower than that in T2DM group（t=13.84,P〈0.05）.Conclusion 1,25（OH）2D3 may up-regulate the expression of VDR gene and then up-regulate the expressions of AdipoR2 and PPAR-αthat decrease the content of TG in the liver.