鸭梨褐腐病菌的分离纯化及其致腐性研究

The separation, identification and corrmption of browning rotten pathogens in Ya-li pears

以河北廊坊九州具有典型褐腐病的鸭梨为研究对象,对引起鸭梨贮期褐腐病的病原菌进行了分析与鉴定,并研究了其致腐性。通过改良后PDA培养基对病果表面的微生物进行分离,共分离纯化得到不同菌落形态的4株菌株,分别为S1、S2、S3、S4,通过科赫法则回接到健康鸭梨,进一步研究其致腐性。结果表明,这4株菌株对鸭梨的致腐效果存在明显差异,其S1和S4致腐性能最强,从而表现出导致鸭梨腐烂变质能力存在差异,然后对这2株微生物结合16s rRNA基因序列分析,最终鉴定S1为桃褐腐病菌、S4为黄曲霉菌。

After Ya-li pears with browning rotten disease in Jiuzhou of Langfang Hebei were taken as materials, the pathogens of browning rotten diseases were separated and identificated, and then their rotten abilities were studied. The pathogens of the rotten pears were separated with improved potato culture medium, and 4 kinds of microorganisms were obtained, which were numbered S1, S2, S3, S4. Then the separated microorganisms were given back to the healthy Ya-li pears by Koch rules, which could cause the diseases at different degrees. Number S1 and number S4 with strongest degrees caused rotten disease, and then the rotten degree were different. Therefore, number S1 and number S4 microorganisms were analysed with 16 s rRNA gene sequence analysis. And the number S1 and number S4 microorganisms were proved that number S1 was Monilinia fructicola, and number S4 was Aspergillus flavus.