同源盒蛋白B7和细胞增殖核抗原在胆管癌组织的表达及其临床意义

The expression and clinical significance of homeobox B7 and proliferation cell nuclear antigen in cholangiocarcinoma

目的探讨同源盒蛋白B7（HOXB7）与细胞增殖核抗原（Ki-67）在胆管癌组织中的表达并分析其临床意义。方法采用免疫组织化学技术检测HOXB7与Ki-67在70例胆管癌组织和15例非肿瘤胆道上皮组织中的阳性表达率，分析HOXB7和Ki-67表达与胆管癌临床进展和预后的关系及表达相关性。结果70例胆管癌、15例非肿瘤胆道上皮组织中HOXB7与Ki-67阳性表达率分别为57.1%、20.0%；71.4%、26.7%，均明显高于非肿瘤胆道上皮组织（χ^2＝6.818，P＝0.009；χ^2＝10.682，P＝0.001）。Ⅲ＋Ⅳ期胆管癌组织中HOXB7和Ki-67表达阳性率明显高于Ⅰ＋Ⅱ期（χ^2＝4.073，P＝0.044；χ^2＝4.488，P＝0.034）；中低分化组HOXB7和Ki-67阳性表达率明显高于高分化组（χ^2＝7.807，P＝0.020；χ^2＝8.270，P＝0.016）；淋巴结转移组中HOXB7和Ki-67蛋白表达阳性率明显高于无淋巴结转移组（χ^2＝6.076，P＝0.014；χ^2＝4.667，P＝0.031）。HOXB7与Ki-67表达呈显著正相关（r＝0.283，P＝0.018）。随着HOXB7和Ki-67表达水平的增加，胆管癌患者术后中位生存期明显缩短。结论HOXB7与Ki-67高表达与胆管癌的发生发展密切相关。

Objective To investigate the expression of homeobox B7 （HOXB7） and proliferation cell nuclear antigen （Ki-67） in cholangiocarcinoma tissues and the clinical significances.Methods Immunohistochemical streptavid-in-peroxidase （SP） method was used to detect the expression of HOXB7 and Ki-67 in 70 cases of cholangiocarcinoma and 15 cases of non-tumor bile duct epithelium. The relationship between the expression of HOXB7 and Ki-67 and clinical progression and prognosis was analyzed. The correlation between the expression of HOXB7 and Ki-67 was analyzed by Spearman analysis method.Results The positive expression rate of HOXB7 and Ki-67 in cholangiocarcinoma tissue （57.1% and 71.4%） was significantly higher than that in non-tumor bile duct epithelium （20.0% and 26.7%; χ^2=6.818, P=0.009; χ^2=10.682, P=0.001）. HOXB7 and Ki-67 expression was significantly correlated with clinical stage, tumor grade and lymph node metastasis. The positive expression rate of HOXB7 and Ki-67 was significantly higher in the stage Ⅲ＋ Ⅳ group （χ^2=4.073, P=0.044; χ^2=4.488, P=0.034）, in moderately or poorly-differentiated group than that in well-differentiated group （χ^2=7.807, P=0.020; χ^2=8.270, P=0.016） and in lymph node metastasis than that in no lymph node metastasis （χ^2=6.076, P=0.014; χ^2=4.667, P=0.031）. A significant correlation was found between the expressions of HOXB7 and Ki-67 in cholangiocarcinoma （r=0.283, P=0.018）. The post-operative mean survival time of patients was shortened with the increased expression levels of HOXB7 and Ki-67.Conclusion The abnormal expression of HOXB7 and Ki-67 may play an important role in the occurrence and development of cholangiocarcinoma.