性别决定基因相关HMG盒基因4和细胞核增殖抗原在胃癌组织的表达及临床意义

The expression of sex determining region Y - box 4 and proliferation cell nuclear antigen in gastric cancer tissue and its clinical significance

目的观察胃癌组织中性别决定基因相关HMG盒基因4（SOX4）和细胞核增殖抗原（Ki-67）的表达，探讨SOX4和Ki-67在胃癌组织表达的临床意义。方法采用免疫组织化学方法检测60例胃癌组织及60例其邻近正常胃组织中SOX4和Ki-67的表达，分析其在胃癌组织中的表达与临床病理特征间的关系。结果SOX4在胃癌组织中呈高表达，阳性表达率为71.7%（43/60），在正常胃组织中未见SOX4表达，Ki-67在胃癌组织中呈高表达，阳性表达率为86.7%（52/60），两者呈明显正相关（r＝0.615, P＝0.003）；SOX4和Ki-67在胃癌组织中的表达与患者年龄、性别、肿瘤大小、浸润深度无明显相关（χ^2＝0.056，P＝0.821；χ^2＝0.572，P＝0.461；χ^2＝0.131，P＝0.882；χ^2＝1.479，P＝0.223；χ^2＝0.652，P＝0.482；χ^2＝0.567，P＝0.512；χ^2＝0.545，P＝0.652；χ^2＝0.781，P＝0.691），SOX4和Ki-67表达与肿瘤分化程度、淋巴结转移、TNM分期明显相关（χ^2＝6.522，P＝0.016；χ^2＝7.681，P＝0.005；χ^2＝9.205，P＝0.003；χ^2＝6.761，P＝0.012；χ^2＝5.521，P＝0.032；χ^2＝5.857，P＝0.017），SOX4和Ki-67在低分化、淋巴结转移、Ⅲ～Ⅳ期胃癌组织中的表达水平明显增高。结论SOX4和Ki-67在胃癌组织中呈高表达，参与胃癌的发生、发展，两者之间密切相关。

Objective To study the expression of sex determining region Y-box 4 （SOX4） and proliferation cell nuclear antigen （Ki-67） in gastric cancer and investigate the clinical significance with the expression of SOX4 and Ki-67 in gastric cancer.Methods Immunohistochemistry mothod was used to detect the expression of SOX4 and Ki-67 in gastric cancer in 60 gastric cancer tissues and 60 nearby normal gastric tissues, the relationship between the expression of SOX4, Ki-67 and the clinicopathological parameters was analyzed.Results The SOX4 highly express in the gastric cancer tissue and the positive rate of SOX4 expresssion in gastric cancer was 71.7% （43/60）, no expression of SOX4 was detected in normal gastric mucosa.The Ki-67 highly express in gastric cancer in the gastric cancer tissue, and the positive rate of the Ki-67 expression in gastric cancer was 86.7% （52/60）. Both the expression of SOX4 and the expression of Ki-67 in gastric cancer were positively associated （r=0.615, P=0.003）; The expressions of SOX4, Ki-67 in gastric cancer were not related to the age, gender, tumor size and the tumor infiltration depth （χ^2=0.056, P=0.821; χ^2=0.572, P=0.461; χ^2=0.131, P=0.882; χ^2=1.479, P=0.223 and χ^2=0.652, P=0.482; χ^2=0.567, P=0.512; χ^2=0.545, P=0.652; χ^2=0.781, P=0.691）. The expressions of SOX4 and Ki-67 were positively associated with histological differentiation, lymph node metastasis and clinical stage （χ^2=6.522, P=0.016; χ^2=7.681, P=0.005; χ^2=9.205, P=0.003 and χ^2=6.761, P=0.012; χ^2=5.521, P=0.032; χ^2=5.857, P=0.017）in gastric cancer.Conclusion SOX4 and Ki-67 were highly expressed in gastric cancer, they may play important roles in the tumorigenesis and progression of gastric cancer and there is a close relationship between them.