双波长分光光度法测定药物及生物样品中呋塞米

Determination of Furosemide in Drug and Biological Samples by Dual Wavelength Spectrophotometry

在pH=3.0~6.5的Tris-HCl缓冲溶液中,呋塞米与维多利亚蓝B(VB)反应生成具有明显正、负吸收峰的离子缔合物,其最大正吸收波长位于625nm,最大负吸收波长位于655nm,采用双波长叠加法测定,表观摩尔吸光系数ε为2.46×104 L/(mol·cm),检出限为0.045mg/L,呋塞米的质量浓度在0.27~5.0mg/L范围内服从比尔定律。方法用于市售呋塞米药物、人体血液及尿液中呋塞米含量的测定,回收率为97.9%~104%,相对标准偏差为1.8%~2.5%。

In Tris-HC1 buffer solution of pH=3.0-6.5,furosemide（FUR） reacts with Vitoria blue B to form an ion association complex with obvious positive absorption peak and negative absorption peak. The maximum positive absorption wavelength locates at 625 nm and the maximum negative absorption wavelength locates at 655 nm. When the double wavelength superposition absorption spectrometry was used to determine the concentrations of furosemide,its apparent molar absorptivity（c） was 2.46 X 10x4 L/（mol· cm） and the detection limit of furosemide was 0. 045 mg/L. Lombard Beer＇s law was obeyed in the range of 0. 27-5.0 mg/L. The method was applied to determine the content of furosemide in commercially available furosemide medicine,human blood and human urine with the recovery and RSD（n=5） in the ranges of 97.9%-104% and 1.80%-2.5% ,respectively.