巨噬细胞移动抑制因子抗体对大鼠大肠异常隐窝病灶及大肠癌的影响

Effect of MIF antibody on aberrant crypt foci and colorectal carcinoma in rat

目的探讨巨噬细胞移动抑制因子(MIF)抗体对二甲基肼诱导的大鼠大肠异常隐窝病灶(ACF)、癌瘤数目及大肠癌变过程中MIF表达的影响。方法二甲基肼诱导建立大鼠大肠癌癌变模型,观察MIF抗体对ACF及癌瘤数量的抑制作用,并采用ELISA及免疫组化方法检测MIF抗体对早期癌变肠黏膜及癌瘤形成后MIF表达的影响。结果 MIF抗体干预后可明显抑制ACF及癌瘤的数量(P<0.01)。大鼠大肠癌模型中MIF的表达明显高于癌前病变ACF模型中MIF的表达(P<0.01),应用MIF抗体可明显抑制大鼠ACF及大肠癌模型中MIF表达。结论 MIF抗体可明显抑制大鼠大肠黏膜癌变,可能与抑制ACF数量及MIF表达有关,MIF抗体有望成为大肠癌预防和治疗的新靶点。

Objective To study the effect of macrophage migration inhibitory factor（MIF） antibody on the rat colonic aber- rant crypt foci（ACF） induced by 1,2-dimethylhydrazine（DMH）, carcinoma number and expression of MIF in rat colonic carcino- genesis. Methods Rat colonic carcinogenesis model was induced by DMH. In this model,the inhibitory effect of MIF antibody on the number of ACF and carcinoma was observed. ELISA and immunohistochemical stainigg were adopted to investigate the effect of MIF antibody in early cancerative intestinal mucosa and MIF expression after cancer formation. Results The number of ACF and carcinoma was significantly inhibited by MIF antibody intervention （P~ 0.01）. The expression of MIF in the colonic carcinoma model was significantly higher than that in the pre-carcinoma ACF model（P（0.01）, Applying MIF antibody could significantly in- hibit the expression of MIF in both rat colonic ACF and colonic carcinoma model, Conclusion MIF antibody can significantly inhibit the rat colonic mucosal carcinogenesis,which may be related with inhibiting number of ACF and expression of MIF. MIF antibody may be expected to become a new target spot of precaution and treatment of colonic carcinoma.