pSS患者血清TNF-α、TGF-β1水平与唇腺淋巴细胞浸润程度及ILD的相关性研究

Correlation of serum level of TNF-α and TGF-β1 with infiltration degree of lymphocytes in labial glands and interstitial lung disease in patients with primary Sjogren′s syndrome

目的探讨原发性干燥综合征(pSS)患者外周血清中肿瘤坏死因子α(TNF-α)、转化生长因子β1(TGF-β1)表达水平与患者唇腺淋巴细胞浸润程度及肺间质病变(ILD)的相关性。方法采用酶联免疫吸附测定法(ELISA)测定116例pSS患者及20例健康体检者(对照组)血清TNF-α、TGF-β1水平,同时对pSS患者唇腺标本进行病理检测。根据肺间质纤维化病变分期及唇腺病理分级将116例pSS患者分为单纯pSS组(n=68)、pSS-ILD组(n=48)及无浸润组(n=36)、浸润组(n=80),对血清TNF-α、TGF-β1水平进行统计学分析。结果 pSS-ILD组患者血清TNF-α、TGF-β1水平明显高于单纯pSS组和对照组(P<0.01),单纯pSS组患者血清TNF-α、TGF-β1水平明显高于对照组(P<0.01);唇腺淋巴细胞浸润组患者血清TNF-α、TGF-β1水平明显高于无唇腺淋巴细胞浸润组和对照组(P<0.01),无唇腺淋巴细胞浸润组患者血清TNF-α、TGF-β1水平明显高于对照组(P<0.01)。TNF-α、TGF-β1表达与淋巴细胞浸润灶分级及肺间质纤维化病变分期呈正相关(r=0.867、0.613、0.814、0.864,P=0.000);TNF-α与TGF-β1的相对表达量呈正相关(r=0.857,P=0.000)。结论 TNF-α、TGF-β1参与了pSS患者唇腺淋巴细胞浸润及ILD的发生、发展,二者表达增加可能是pSS患者唇腺结构破坏及合并ILD的原因。

Objective To investigate the correlation between peripheral serum TNF-a and TGF-β1 expression levels with the infiltration degree of lymphocytes in labial glands and interstitial lung disease（ILD） in the patients with primary Sjogren＇s syndrome （pSS）. Methods Serum level of TNF-a and TGF-β1in 116 patients with pSS and 20 persons undergoing physical examination were measured by ELISA. Meanwhile the labial gland samples in pSS patients were performed the pathological examination. The patients were grouped according to the lesion stage of ILD and pathology grade of labial glands. The serum TNF-a and TGF-β1 levels were statistically analyzed. Results Serum levels of TNF-a and TGF-β1 in the pSS -ILD group were significantly higher than those in the simple pSS group and control group（P〈0.01）. The levels of serum TNF-a and TGF-β1in the simple pSS group were significantly higher than those in the control group（P〈0.01）. The levels of serum TNF-a and TGF-β1in the labial glands lymphocytes ihfihra- tion group were significantly higher than those in the labial glands lymphocytes non-infiltration group and control group（P〈0.01）, while the levels of serum TNF-a and TGF-β1 in the labial glands lymphocytes non-infiltration group were significantly higher than those in the control group（P〈0.01）. The TNF-a and TGF-β1 expression was positively correlated with lymphocytes infiltration le- sion grade（r=0. 867,0. 613, P= 0. 000） ;the expression of TNF-a and TGF-β1 was positively correlated with the lesion grade of ILD（r= 0. 814,0. 864, P = 0. 000）;the level of TNF-a was positively correlated with the TGF-β1 relative expression amount （r = 0. 857 ,P=0. 000）. Conclusion TNF-a and TGF-β1 are involved in the lymphocytes infiltration of labial glands and ILD occurrence and develvp meat,and their expression increase may be the cause of labial glands structure destruction and complicating ILD.