急腹症Ⅲ号对呼吸机相关性肺炎大鼠小肠形态的影响

Effect of acute abdomen No.Ⅲ on small intestine morphology of rats with ventilator-associated pneumonia

目的观察急腹症Ⅲ号对呼吸机相关性肺炎模型大鼠小肠形态的影响,并探讨其作用机制。方法雄性SD大鼠100只,随机分为五组:急腹症Ⅲ号组(VAP模型,急腹症Ⅲ号灌胃)、谷氨酰胺组(VAP模型,谷氨酰胺颗粒灌胃)、急腹症Ⅲ号联合谷氨酰胺组(VAP模型,急腹症Ⅲ号及谷氨酰胺颗粒灌胃)、安慰剂组(VAP模型,生理盐水灌胃)、空白对照组(生理盐水灌胃)。术后第5天收集尿液,采用L/M(乳果糖/甘露醇法)评价肠黏膜通透性,术后第6天处死大鼠取小肠进行病理检测。结果安慰剂组L/M较空白对照组明显增大(P<0.01),其余三个干预组与空白对照组相似。安慰剂组小肠绒毛高度、黏膜厚度及全层厚度均低于空白对照组(P<0.01)。三个干预组小肠绒毛高度与空白对照组相似(P>0.05),且均高于安慰剂组(P<0.01),联合组绒毛高度高于急腹症Ⅲ号组及谷氨酰胺组(P<0.01),但急腹症Ⅲ号组与谷氨酰胺组之间差异无统计学意义(P>0.05)。空白组黏膜厚度与急腹症Ⅲ号组及联合组相似(P>0.05),但高于谷氨酰胺组(P<0.05),三个干预组均高于安慰剂组(P<0.01),但三组之间无差异(P>0.05)。联合组全层厚度高于安慰剂组(P<0.01),其余组之间无统计学差异(P>0.05)。结论急腹症Ⅲ号可减少呼吸机相关性肺炎大鼠模型肠黏膜通透性增加,减少小肠黏膜结构的破坏,保护肠黏膜结构。

Objective To observe the effect of acute abdomen No.Ⅲ on small intestine morphology in rats with ventila- tor-associated pneumonia and to explore its mechanism. Methods A total of 100 male SD rats were randomly divided into 5 groups. Acute abdomen No. Ⅲ group： VAP model, acute abdomen No. Ⅲ gavage; glutamine group： VAP model, glutamine granules gavage; acute abdomen No. Ⅲ combined with glutamine group： VAP model, acute abdomen No. Ⅲ and glutamine .granules gavage; placebo group： VAP model, normal saline gavage; blank control group： normal saline gavage. Urine was collected on the 5th day after surgery. L/M （lactulose/mannitol method）was used to evaluate the in- testinal mucosal permeability. The rats were sacrificed on the 6th day after surgery to Collect the small intestine for pathological examination. Results L/M in placebo group was significantly higher than that in blank control group（P〈0.01）, and L/M in the bther three intervention groups were similar to that in the blank control group. The small intestine villi height, mucosal thickness and full thickness in the placebo group were all lower than those in the blank control group （P〈0.01）. The small intestine villi height in the three intervention groups was similar to that in the blank control group （P〉0.05）, and were all higher than that in the placebo group（P〈0.01）. The villus height in the combined group was higher than that in the acute abdomen No. In group and glutamine group（P〈0.01）. However, there was no difference between a- cute abdomen No.Ⅲ group and glutamine group（P〉0.05）. The mucosal thickness in the blank group was similar to that in the acute abdomen No.Ⅲ group and the combined group（P〉0.05）, but higher than glutamine group（P〈0.05）. The three intervention groups were higher than placebo group（P〈0.01）. However, there was no difference between the three groups（P〉0.05）. The total thickness in the combined group was higher than that in the placebo group（P〈0.01）, there was no statistically significant difference between the other groups（P〉0.05）. Conclusion Acute abdomen No. m can re- duce the increase of intestinal mucosal permeability in rats model with ventilator-associated pneumonia, reduce the de- struction of intestinal mucosal structure and protect the structure of intestinal mucosa.