摘要
以‘春绿兰×虎雪兰’F_1代的原球茎为外植体,在无菌条件下,以1/2MS培养基为基本培养基,添加不同浓度6-BA与NAA,研究了不同激素配比对原球茎不同培养阶段的增殖、分化和生根的影响。结果表明:1/2MS+1.0 mg·L^(-1) 6-BA+0.5 mg·L^(-1)NAA+80g·L^(-1)香蕉泥,利于原球茎的增殖和分化,增殖率和分化率分别为222.00%、90.57%;1/2MS+1.5mg·L^(-1) 6-BA+0.5mg·L^(-1) NAA+80g·L^(-1)香蕉泥,利于不定芽增殖,增殖率为42.50%;1/2MS+0.5mg·L^(-1) 6-BA+1.5mg·L^(-1) NAA生根效果最佳,生根率为100%,平均株根数为5.50条,平均根长为40.73mm,且植株长势健壮。
The Cymbidium faberi Rolfe var.Szechuanicum ×(C.tracyanum var.Huanghua×C.mastersii)F_1 hybrids as test material,protocorm-like-body(PLB)were used as explants in tissue culture to study the influence of BA and NAA in different concentrations,mashed bananas and activated carbon on their regeneration under 1/2 MS basic medium.The results showed that 1/2 MS+1.0 mg·L^(-1) 6-BA+0.5 mg·L^(-1) NAA+80 g·L^(-1) mashed bananas could make PLB rate to 222.00% and differentiation rate to 90.57% ,1/2 MS+1.5 mg·L^(-1) 6-BA+0.5 mg·L^(-1) NAA+80 g·L^(-1) mashed bananas could make multiplication rate to 42.50% ,1/2 MS+0.5 mg·L^(-1) 6-BA+1.5 mg·L^(-1) NAA could make rooting rate to 100% ,the average number of plant roots was 5.50,average root length was 40.73 mm,and plant grew healthily.
出处
《北方园艺》
CAS
北大核心
2018年第3期87-91,共5页
Northern Horticulture
基金
云南省重点新产品开发资助项目(2012BB008)
云南省昆明市科学技术局重点资助项目(2015-1-N-00984)
关键词
原球茎
增殖培养
分化率
生根培养
protocorm-like-body (PLB)
multiplication culture
differentiation rate
rooting culture