弓形虫感染对人精液参数和精子DNA完整性的影响

Effects of Toxoplasma gondii on human semen parameters and sperm DNA integrity

目的弓形虫(TOX)感染与男性不育有关,精子DNA完整性是反映男性生育能力的重要标志物,本文探讨TOX感染对人精液参数和精子DNA完整性的影响。方法选择2014年7月—2016年5月在浙江中药大学附属宁波中医院男科就诊的不育患者156例,根据抗弓形虫抗体结果分为TOX-IGG阳性组、TOX-IGM阳性组、其他感染组和未发生感染组;选择同期健康男性作为对照组。分析各组精液质量参数和精子DNA碎片化指数[DFI(%)]的差异。精子浓度及活力用计算机辅助精子分析系统,精子存活率采用低渗膨胀实验,精子形态用DiffQuick法,精子DFI用单细胞凝胶电泳(SCGE)法。结果各不育组精液质量参数与对照组比较差异均有统计学意义(P<0.05);TOX感染不育组前向运动精子较未发生感染不育组明显减少(P<0.05)。TOX感染不育组各DFI较对照组差异均有统计学意义(P<0.05);较未发生感染不育组轻度DFI差异无统计学意义(P>0.05),重度和总DFI差异均有统计学意义(P<0.05);较其他感染不育组各DFI差异均无统计学意义(P>0.05)。TOXIGM阳性不育组较TOX-IGG阳性不育组重度和总的DFI显著增多(P<0.01)。结论 TOX感染可影响人类精液参数和损伤精子DNA的完整性,并且新近感染损伤程度大于既往感染。

Objective Toxoplasma gondii（TOX） infection is associated with male infertility. Sperm DNA integrity is an important marker of male fertility. The aim of this paper is to investigate the influence of TOX infection on the human se- men parameters and sperm DNA integrity. Methods The recruited infertile patients were divided into TOX-IGG positive group, TOX-1GM positive group, other pathogens infection group and no-infection group. The recruited normal males with normal fertility were included as controls. The differences in semen quality and sperm DNA fragmentation index （DFI） were statistically analyzed between the infertile men and normal controls. The sperm concentration and vitality were ana- lyzed by using computer assisted sperm analysis（CASA） , sperm survival rate by using hypoosmotic swelling test, sperm morphology by using Diff-Qick staining, and sperm DNA damage index DFI（ % ） by using single cell gel electrophoresis （SCGE）. Results There were statistically significant differences in semen quality parameters between the control and the infertile groups （P 〈 0.05 ）. Compared with the no-infection group, the rate of progressively motile sperm （PR） in TOX infection group was significantly decreased（ P 〈 0.05 ）. The differences in mild DFI, severe DFI and total DFI were statistically significant between the TOX infection groups and normal controls. Compared with the no-infection group, the severe and total DFI in TOX infection group had statistically significant differences（P 〈0.05）, mild DFI in TOX infec- tion group had no significant differences（ P 〉 0.05 ）. Compared with the other pathogens infection group, the mild, se- vere and total DFI in TOX infection group had no statistically significant differences（ P 〉 0.05 ）. Compared with the TOX- IGG positive group, the severe and total DFI in TOX-IGM positive group were significantly increased（P 〈 0.01 ）. Con- clusion TOX infection could affect human semen parameters and sperm DNA integrity. The degree of damage in new in- fections was more obvious compared with the past infections.