基于不同频率摩腹法干预对脾虚型家兔十二指肠黏膜细胞病理差异分析

Pathological difference analysis of duodenum mucosa cells in rabbits with spleen-deficiency syndrome based on different frequencies abdomen rubbing manipulation

目的:通过分析不同频率摩腹法干预下脾虚型家兔十二指肠黏膜细胞病理差异,从病理学角度探讨不同频率摩腹法对脾虚型家兔的作用机制。方法:将48只家兔随机分组,分别为A、B、C、D、P、K 6组,每组8只。其中K组为空白对照组,其余组采用苦寒泻下法制作脾虚模型,以不同频率摩腹法对A组(50-100次/min)、B组(101-150次/min)、C组(151-200次/min)、D组(201-250次/min)4组进行为期10d的手法干预,P组作为模型对照组,手法结束后家兔空腹1d再进行处死和解剖,截取胃肠标本制片放光镜下观察十二指肠黏膜细胞病理变化。结果:光镜下示K组家兔十二指肠黏膜细胞形态规则、层次清晰、结构完整;P组黏膜上皮细胞坏死脱落明显,大量炎性细胞浸润,绒毛结构破坏。手法干预的4组家兔从黏膜厚度、吸收细胞大小、十二指肠腺数量、炎细胞数量、血管数量、杯状细胞数量6个方面综合分析,得出十二指肠黏膜细胞有不同程度的再生和重构。不同频率摩腹法可以有效地缓解脾虚型家兔病理学改变,改善并修复苦寒泻下药液对胃肠黏膜的损伤。其中手法频率在101-150次/min的摩腹法效果最佳,频率在201-250次/min效果较差。结论:摩腹法具有缓解胃肠黏膜损伤、保护肠胃黏膜的作用。

Objective：Through analyzing the pathological differences of duodenal mucosa in rabbits with spleendeficiency syndrome under the intervention of different frequencies abdomen rubbing manipulation,to explore the mechanism of different frequencies abdomen rubbing manipulation on rabbits with spleen-deficiency syndrome from the perspective of pathology.Method：A total of 48 rabbits were randomly divided into six groups of A,B,C,D,P and K,with 8 in each group.Among them,group K was the blank control group,and the remaining groups were treated with bitter cold diarrhea method to make spleen-deficiency model.Group A was with rubbing method（50-100 times/min）,group Bwas withrubbing method（101-150 times/min）,group Cwas withrubbing method（151-200 times/min）,and group D was withrubbing method（201-250 times/min）,four groups taken ten days of abdomen rubbing manipulation.Group P was the model control group.After the end of the manipulation and fasting for 1 day,rabbits were sacrificed and dissected,and gastrointestinal specimens were made to observe pathological changes in duodenal mucosal cells by light microscopy.Results：Light microscope showed that：the morphology of duodenal mucosa in K group was clear and the structure was intact; The mucosal epithelial cells in group P showed obvious necrosis and shedding,with a large number of inflammatory cell infiltration and villus structure destruction.The four groups of rabbits intervening with manipulation were analyzed comprehensively in terms of mucosal thickness,cell size,number of duodenum gland,number of inflammatory cells,number of blood vessels and number of goblet cells.The results showed that duodenal mucosal cells had different degrees of regeneration and reconstruction.Different frequency rubbing abdomen manipulation method can effectively alleviate the pathological changes of spleen-deficiency rabbits to improve and repair the bitter cold diarrhea liquid medicine on the gastrointestinal mucosal injury.The effect of frequency of the 101-150 times/min is best,while the effect of frequency of 201-201 times/min of the abdominal rubbing manipulation is poorer.Conclusion：The mordant method has the effects of relieving gastrointestinal mucosa injury and protecting gastrointestinal mucosa.