天王补心丹对慢性睡眠剥夺模型大鼠心肌、下丘脑视交叉上核VIP、AVP表达的影响

Effects of Tianwang Buxin Dan on VIP and AVP Expressions of Model Rats with Chronic Sleep Deprivation

目的 ：探讨天王补心丹对慢性睡眠剥夺模型大鼠药物干预的可能机制。方法：40只雄性SD大鼠随机分为空白组、模型组、天王补心丹组和艾司唑仑组。采用多平台水环境联合咖啡因注射复制慢性睡眠剥夺模型。造模完成后用天王补心丹和艾司唑仑对模型大鼠进行治疗。以行为学测试检测大鼠学习记忆水平,取心肌和下丘脑视交叉上核（SCN）观察形态学改变,ELISA法检测VIP、AVP的表达水平。结果：行为学测试：与模型组比较,治疗组大鼠对位训练与探查训练结果有显著性差异（P〈0.05,P〈0.01）。透射电镜：与模型组比较,治疗组大鼠心肌组织及SCN损伤程度减轻。ELISA：与模型组比较,治疗组大鼠心肌中VIP、AVP的表达下调（P〈0.05,P〈0.01）,SCN中VIP、AVP的表达上调（P〈0.05,P〈0.01）。结论：天王补心丹“心脑相关”的药物干预机制可能和调节心肌、SCN中VIP、AVP的表达有关。

Objective ： To explore possible mechanism of pharmacological intervention of Tianwang Buxin Dan on model rats with chronic sleep deprivation （CSD）. Methods：Forty male rats were randomly divided into blank control group, mod- el group, Tianwang Buxin Dan group, Estazolam group. The CSD rats models were established by multiple platform water environment combined with caffeine injection and were treated with Tianwang Buxin Dan and Estazolam. Results：Behav- ioral test： Compared with the model group, the treatment groups had significant difference between counterpoint training and exploration training（ P 〈 0. 05, P 〈0. 01 ）. Transmission electron microscope： Compared with the model group, the damage degree of myocardial tissue and SCN were decreased in the treatment groups. ELISA： Compared with the model group, the expressions of VIP and AVP in the myocardium was down regn!ated, and that in SCN was up regulated in the treatment groups（P 〈0. 05, P 〈0. 01 ）. Conclusion： The mechanism of cardiac and cerebral correlation might be possibly related to regulating the expressions of VIP and AVP in myocardium and SCN.