摘要
目的:研究沉默Cx43基因对左归丸含药血清促MC3T3-E1细胞分化作用的影响。方法:采用慢病毒介导的RNA干扰技术沉默Cx43基因,将培养细胞分为正常对照A组、含药血清B组、基因沉默C组。细胞培养第3、5、7、9天进行ALP活性测定、第21天矿化结节茜素红染色,进行组间对比。结果:左归丸含药血清可以促进MC3T3-E1细胞的分化,与A组比较:ALP活性增强,其中第5、7、9天差异显著(P〈0.05);茜素红染色面积增加(P〈0.01)。C组与B组比较:ALP活性降低,其中第7、9天差异显著(P〈0.05);茜素红染色面积明显减少(P〈0.05)。结论:沉默细胞Cx43基因导致左归丸含药血清促MC3T3-E1细胞分化作用明显下降。
Objective: To study the effect of Cx43 gene silencing on the promoting differentiation of MC3T3 -E1 cells induced by Zuogui Pill medicated serum. Methods : First, lentivirus - mediated RNA interference technique was used to silence the Cx43 gene and then the cultured cells were divided into normal control group A, serum group B and gene silen- cing group C. ALP activity was measured and compared at 3,5,7 and 9 days, and mineralized nodules by alizarin red stai- ning were detected and compared at day 21. Results: Zuogui Pill medicated serum can promote the differentiation of MC3T3 - E1 cells. Compared with A group, ALP activity increased, of which the 5,7 and 9 days were significantly differ- ent (P 〈0. 05) ;and the alizarin red staining area increased(P 〈0. 01 ). C group compared with B group,ALP activity decreased, and at 7 and 9 day were significantly different (P 〈 0. 05 ) ;and the alizarin red staining area was significantly reduced (P 〈0. 05 ). Conclusion: Zuogui Pill medicated serum on MC3T3 - E1 cell promoting differentiation was signifi- cantly decreased by silencing of Cx43 gene.
作者
桑红灵
周安方
刘健
陈好远
章程鹏
萧闵
SANG Hongling;ZHOU Anfang;LIU Jian;CHEN Haoyuan;ZHANG Chengpeng;XIAO Min(Hubei University of Traditional Chinese Medicine, Wuhan 430061, Hubei, China;Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第2期399-402,I0022,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81403285)
国家中医药管理局“周安方全国名老中医药专家传承工作室”建设项目([2014]20)