脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制

The combined mechanism of fatty acid translocase FAT/CD36 and long chain fatty acid

目的 探讨脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制。 方法 ELISA分析重组蛋白FAT/CD36与不同种类长链脂肪酸结合强弱机制,分子模拟对接进一步验证其结合机制并分析其可能作用位点。 结果ELISA证明重组蛋白FAT/CD36与不同种类长链脂肪酸均显示特异性结合,结合值大小为油酸＞棕榈酸＞棕榈油酸＞肉豆蔻酸＞硬脂酸。分子模拟对接进一步验证FAT/CD36受体蛋白主要与其中的油酸、棕榈酸及棕榈油酸结合,且结合位点位于FAT/CD36受体蛋白胞外结构的Arg183-Lys-Gly-Lys-Arg-Asn-Leu-Ser-Tyr238区域组成的活性口袋。 结论 FAT/CD36作为细胞长链脂肪酸转运的主要受体,其可能主要转运油酸、棕榈酸和棕榈油酸这三种脂肪酸。

Aim To investigate the binding mechanism of fatty acid translocase FAT/CD36 and long chain fatty acid. Methods ELISA was used to analyze the intensity mechanism of the purity of FAT/CD36 binding with different fatty acid, molecular simulation docking was used to further verify the binding mechanism and analyze its possible binding sites. Results ELISA proved that the recombinant protein FAT/CD36 with different types of long chain fatty acids showed specific binding, binding values for oleic acid〉palmitic acid〉palmitoleic acid〉myristic acid〉stearic acid. Molecular simulation docking further confirmed that FAT/CD36 receptor protein mainly combines with oleic acid, palmitic acid and palmitoleic acid. And the binding site was located in the extracellular structure of the FAT/CD36 receptor protein Arg183-Lys-Gly-Lys-Arg-Asn-Leu-Ser-Tyr238 region consisting of the active pocket. Conclusion FAT/CD36, as the main receptor of long chain fatty acid transport, may transport oleic acid, palmitic acid and palmitoleic acid.