芳香外敷方对肺癌骨转移模型小鼠转移瘤体生长的影响

Impact of Fangxiang Waifu Fang ( 芳香外敷方) on growth of Metastatic Tumor of Model Mice with Bone Metastasis of Lung Cancer

目的观察中药芳香外敷方对肺癌骨转移癌的作用。方法通过股骨内注射转染绿色荧光蛋白的人肺腺癌A549细胞建立肺癌骨转移癌小鼠模型,造模成功后随机分为中药外敷组[芳香外敷方0.5 g/(kg·d)局部外敷,造模后7~21天每日1次]、唑来膦酸组[造模后7、10、14、19天腹腔注射唑来膦酸注射液30μg/(kg·d)]、模型组(生理盐水局部外涂,0.5 ml/只,每日1次),每组10只,另设假手术组(生理盐水局部外涂,0.5 ml/只,每日1次)10只。于造模当日及造模后第3、7、11、14、17、21天分别测量各组小鼠体质量,并采用FluorVivo成像系统观察小鼠肿瘤面积及转移情况。于造模21天称量瘤体重量。结果各组小鼠不同时间体质量比较差异均无统计学意义(P>0.05)。各组小鼠未出现其他部位和器官的转移。第21天中药外敷组小鼠肿瘤面积为(12.32±6.94)mm^2,小于模型组的(23.91±9.86)mm^2及唑来膦酸组的(17.16±7.37)mm^2(P<0.05),且瘤体重量亦小于模型组(P<0.01)。唑来膦酸组与模型组比较,肿瘤面积与瘤体重量差异均无统计学意义(P>0.05)。结论芳香外敷方可抑制肺癌骨转移瘤体增长。

Objective To observe the effect of Chinese medicine Fangxiang Waifu Fang（ 芳香外敷方） on bone metastasis of lung cancer. Methods Established lung cancer with bone metastatic mice models by intra-femur injection human lung adenocarcinoma A549 cells transfected with green fluorescent protein. After successful modeling,mice were randomized into two groups with 10 in each group. Chinese medicine external application group was given Fangxiang Waifu Fang 0. 5 g/（ kg·d） local external application,once every day from 7 th day to 21 st day after modeling,while the zoledronic acid group was given zoledronic acid injection 30 μg/（ kg·d） by intraperitoneal injection on the 7 th,10 th,14 th and 19 th day after modeling,and the model group（ normal saline local external application,0. 5 ml/mouse,once every day）,n = 10. The sham operation group was given normal saline local external application,0. 5 ml/mouse,once every day. Body weight was measured in each group on the modeling day,on the 3 rd,7 th,11 th,14 th,17 th and 21 st day after modeling. Tumor area and metastasis were examined by FluorVivo image-forming system. Tumor weight was measured on the 21 st day of modeling. Results The difference of mice body weight in each group at different time was not significant（ P 0. 05）. No metastasis of other parts and organs was observed in mice in each group. On the 21 st day,mice tumor area was（ 12. 32 ± 6. 94） mm^2 in the Chinese medicine external application group,which was smaller than（ 23. 91 ± 9. 86） mm^2 in the model group and（ 17. 16 ±7. 37） mm^2 in the zoledronic acid group（ P 0. 05）. Moreover,tumor weight was also less than that in the model group（ P 0. 01）. Compared with the zoledronic acid group and the model group,the difference of tumor area and tumor weight was not significant（ P 0. 05）. Conclusion The Fangxiang Waifu Fang could inhibit the tumor growth of lung cancer bone metastasis.