白血病抑制因子受体在宫颈癌肺转移过程中的作用及其机制

Effects of leukaemia inhibitory factor receptor on lung metastasis of cervical cancer

目的探讨白血病抑制因子受体(LIFR)在宫颈癌肺转移中的作用以及Hippo-YAP信号通路在其中的作用。方法 (1)采用实时荧光定量PCR法检测50例宫颈癌肺转移患者原发灶癌组织、癌旁组织及转移灶癌组织LIFR mRNA表达。(2)用包含LIFR的重组慢病毒质粒及其阴性对照空载体质粒vector转染人宫颈癌Si Ha细胞,2.0μg/mL嘌呤霉素筛选,得到稳定过表达LIFR的宫颈癌细胞(观察组)和对照细胞(对照组)。采用Western blotting法检测两组LIFR及Hippo-YAP信号通路相关蛋白YAP、P-YAP、TAZ、p-TAZ。将两组细胞经尾静脉注射到BALB/c裸鼠体内,4周后处死裸鼠,取出肺组织,HE染色,镜下计数肺组织中所有转移灶个数。结果 (1)LIFR mRNA相对表达量:原发灶癌组织高于癌旁组织,转移灶癌组织低于原发灶癌组织和癌旁组织(P均<0.05)。(2)观察组过表达LIFR的Si Ha细胞LIFR、p-YAP、p-TAZ蛋白相对表达量高于对照组(P均<0.05),YAP、TAZ蛋白表达量与对照组比较P均>0.05。注射过表达LIFR的Si Ha细胞及对照细胞的裸鼠肺转移灶个数分别为(9±2)、(60±5)个,P<0.05。结论过表达LIFR可促进Hippo-YAP信号通路蛋白YAP、TAZ磷酸化,进而抑制宫颈癌肺转移灶的形成。

Objective To investigate the role of leukemia inhibitory factor receptor （LIFR） and Hippo-YAP signaling pathway in the lung metastasis of cervical cancer, Methods （1） The real-time fluorescence quantitative polymerase chain reaction （qRT-PCR） was used to detect the expression of LIFR mRNA in the primary cancerous tissues, adjaceni non-can- cerous tissues and metastasis tissues from 50 cases of patients withcervical cancer and lung metastasis. （2） Recombinant lentiviral plasmid containing LIFR or its negative control （vector） were Used to infect the human cervical cancer cell line SiHa. After screening with 2.0 μg/mL puromycin, the cells that stably expressed LIFR （ observation group） or vector （control group） were obtained. Western blotting was used to detect the expression of LIFR and Hippo-YAP signaling path way related proteins YAP, P-YAP, TAZ, and p-TAZ. The cells of the two groups were injected into the BALB/c nude mice through the tail vein. After 4 weeks, the nude mice were killed and the lung tissue was removed for HE staining. The number of all the metastases in the lung tissues was counted microscopically. Results （1） The relative expression of LIFR mRNA in the primary tumor was higher than that in the adjacent non-cancerous tissues, and the relative expression of LIFR mRNA in the metastasis tissues was lower than that in primary tumor and adjacent non-cancerous tissues （ all P 〈 0.05 ）. （2） The relative expression of LIFR, p-YAP and p-TAZ proteins in the SiHa ceils which overexpressed LIFR was higher than that in the control cells （ all P 〈 0.05 ）. There was no significant difference in YAP and TAZ protein expression between the control group and observation group （ all P 〉 0.05 ）. The lung metastases in the SiHa cells with overexpressed LIFR and control cells were 9 ＋ 2 and 60 ＋ 5, respectively （ all P 〈 0.05 ）. Conclusion Overexpression of LIFR can promote the phosphorylation of Hippo-YAP signaling pathway proteins YAP and TAZ, and then inhibit the tormation of lung metastasis of cervical cancer cells.