Ki-67及PTEN在口腔腺样囊性癌中的表达及临床意义

Expression of Ki-67 and PTEN in oral adenoid cystic carcinoma and its clinical significance

目的研究口腔腺样囊性癌(OACC)中Ki-67和PTEN的表达情况,及其表达水平与临床病理特征的关系。方法收集佳木斯大学附属第一医院病理实验室证实的40例口腔腺样囊性癌。另选口腔腺样囊性癌患者标本癌旁正常腺体15例做对照组。免疫组化SP法检测腺样囊性癌中Ki-67蛋白及PTEN的表达。结果 Ki-67蛋白在口腔腺样囊性癌中的阳性率为70%(28/40),明显高于对照组13%(2/15)(P<0.05)。PTEN蛋白在口腔腺样囊性癌中的阳性率为63%(25/40),明显高于对照组20%(3/15)(P<0.05)。Ki-67、PTEN的表达与TNM分期、肿瘤组织学类型、远处转移、淋巴结转移和神经节浸润均显著相关,并且Ki-67和PTEN表达存在相关性。结论 Ki-67和PTEN在口腔腺样囊性癌发生和发展过程中可能起重要作用。Ki-67和PTEN蛋白可能作为口腔腺样囊性癌的诊断标志物。

Objective The aims of this study were to determine the expression of Ki-67 and PTEN in oral adenoid cystic carcinoma（ OACC） and its relationship with clinicopathological features,and to explore the relationship between the expression of Ki-67 and PTEN. Methods Forty cases of oral adenoid cystic carcinoma were collected from the pathological laboratory in our hospital. Another 15 cases of normal gland in patients with oral adenoid cystic carcinoma were selected as the control group. The expression of Ki-67 protein and PTEN in adenoid cystic carcinoma was detected by immunohistochemistry. Results The positive rate of Ki-67 protein in oral adenoid cystic carcinoma was 70%（ 28/40）,which was significantly higher than that in the control group（ 2/15）（ P 0. 05）. The positive rate of PTEN in oral adenoid cystic carcinoma was 63%（ 25/40）,which was significantly higher than that in the control group 20%（ 3/15）（ P 0. 05）. They were associated with histological types,metastasis,lymph node metastasis and neural invasion,and not correlated with age,gender and tumor location. The expression of Ki-67 and PTEN in oral adenoid cystic carcinoma may have a significant correlation.Conclusion The expression of Ki-67 and PTEN in adenoid cystic carcinoma is high,and their occurrence and development in adenoid cystic carcinoma play an important role in the process of evolution and metastasis. Ki-67 and PTEN proteins may be markers of oral adenoid cystic carcinoma.