乳腺癌组织中PDK4的表达及其与预后的关系

PDK4 mRNA Expression in Breast Cancer and Its Relationship with Prognosis

目的探讨影响乳腺癌组织中PDK4的表达与预后的相关性。方法 Pub Med数据库The Gene Expression Omnibus(GEO,http://www.ncbi.nlm.nih.gov/geo/)分析PDK4在正常乳腺及乳腺癌组织中的表达水平。使用The Kaplan–Meier plotter(www.kmplot.com)分析工具评估PDK4表达水平与乳腺癌临床预后的相关性。TGF-β细胞因子处理MCF-7细胞系,Real-time PCR技术检测经处理后MCF-7细胞系中PDK4 m RNA表达水平。si RNA干扰技术降低细胞内PDK4的表达水平,Transwell检测MCF-7细胞的侵袭能力。结果 PDK4在乳腺癌组织中的表达水平低于正常乳腺组织,并且PDK4低表达与乳腺癌预后差呈正相关;在乳腺癌细胞系中,TGF-β降低PDK4 m RNA表达水平,且PDK4的低表达增强了MCF-7细胞的侵袭能力。结论 PDK4低表达是乳腺癌预后差的一个重要指标。

Objective To explore PDK4 mRNA expression in breast cancer tissues and its relationship with prognosis. Methods We analyzed the mRNA expression of PDK4 by the Gene Expression Omnibus （GEO, http：//www.ncbi.nlm.nih.gov/geo/） and estimated the correlation of PDK4 mRNA expression with the prognosis of breast cancer patients by Kaplan-Meier plotter （www.kmplot.com）. Real-time PCR was used to detect the mRNA expression of PDK4 in TGF-β-treated MCF-7 cell line. siRNA decreased the mRNA level of PDK4 in breast cancer cells. Transwell assay was used to detect the invasion ability of MCF-7 cells. Results PDK4 was down-regulated in breast cancer tissues, and the low expression of PDK4 was positively correlated with poor prognosis of breast cancer patients. In breast cancer cell lines, TGF-β decreased PDK4 mRNA expression, and the low expression of PDK4 enhanced the invasion of MCF-7 cells. Conclusion The low mRNA level of PDK4 is an important prognosis indicator for breast cancer.