一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用

Application of Adjustable Gradient Mobile Phase Control System on Biomacromolecule Characterization

以注射泵为基础,通过硬件与软件结合,研制了一种可进行梯度调节的流动相控制系统,并与石英晶体微天平联接,利用该系统对流动相中的盐浓度和pH值分别进行梯度调节,在石英晶体微天平上实时研究了牛血清蛋白（BSA）吸附洗脱的变化.结果表明,该流动相控制系统的流量输出误差为1%,流量稳定性为1%,梯度误差为1%,均符合GB/T 26792-2011要求.随盐浓度增大或pH值降低,BSA逐渐被洗脱,证明该流动相控制系统能精确控制和模拟复杂固液界面.

Based on the syringe pumps and cooperated with the developed software, an adjustable gradient mobile phase control system was developed, furthermore the system was connected with quartz crystal microbalance（QCM）. Through the gradient adjustion of salt concentration or pH value, the real time adsorption and elution variations of bovine serum albumin（BSA） were investigated. The results showed that the flow error was 1%, the flow stability was 1%, and the gradient error was 1%, which met the standard of GB/T 26792-2011. With the increase of salt concentration or decrease of pH value, the BSA was eluted gradually. These results demonstrated that this system could regulate the gradient and simulate complicated solid-liquid interface environment accurately.